Nanoengineered biophotonic hybrid device

ABSTRACT

An improved method for the design and development of high performance hybrid devices having biological and nonbiological components. The biological component is used in hybrid constructs that may be nanostructures, given the small size of the biological parts. In one specific embodiment, chlorosomes of  Chloroflexus aurantiacus  ( C. aurantiacus ) enhance performance of a silicon photovoltaic cell.  C. aurantiacus , strain J-10-f1, has the A.T.C.C. designation number 29366, having been deposited in July, 1976. Its chlorosomes are harvested and positioned in light communicating relation to a photoactive semiconductor. The chlorosomes react to light of a first wavelength by emitting light at a second wavelength to which the semiconductor electrically responds.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a Continuation-in-Part application off of U.S. application Ser. No. 11/475,342 in the name of Jeffrey T. LaBelle and Vincent B. Pizziconi, filed Jun. 26, 2006, entitled “Nanoengineered Biophotonic Hybrid Device,” which claims priority from the divisional U.S. application Ser. No. 10/658,541 in the name of Jeffrey T. LaBelle and Vincent B. Pizziconi, filed Sep. 8, 2003, entitled “Nanoengineered Biophotonic Hybrid Device,” now U.S. Pat. No. 7,067,293, issued Jun. 27, 2006, which claims priority from provisional U.S. patent application Ser. No. 60/408,775, of the same inventors, filed Sep. 7, 2002 entitled “Method & Apparatus for Synthesis, Processing, Design & Manufacturing of High Performance, Scalable & Adaptive, Robust Energy-Interactive Materials, Devices & Systems.” As to subject matter in this application common to one or more of the foregoing three applications, priority from each of the foregoing three applications is hereby claimed. Each of the foregoing three applications is incorporated herein by reference.

This application is related to three concurrently filed applications, each in the name of the present applicants and all sharing priority from the above-identified provisional and parent U.S. utility application Ser. No. 10/658,541. The three concurrently filed applications are entitled “Device With Biological Component And Method Of Making To Achieve A Desired Figure of Merit,” Ser. No. 11/475,343, “Device With Biological Component And Method of Making To Achieve A Desired Transfer Function,” Ser. No. 11,475,338, and “Method Of Making Biological Components For Devices By Forced Environmental Adaptation,” Ser. No. 11/475,356.

STATEMENT OF GOVERNMENT FUNDING

Financial assistance for this project was provided by the U.S. Government through the National Science Foundation Under Grant Numbers 9602258 and 9986614 and the United States Government may own certain rights to this invention.

FIELD OF THE INVENTION

This invention relates to hybrid biological and electronic photosensitive devices and more particularly to nanoscale hybrid devices of this kind and their method of manufacture.

BACKGROUND

Recently, attempts to marry biology and engineering to create various biohybrid constructs have been steadily increasing. A limited number of novel biohybrid sensor applications have already been reported, and in some cases commercialized, that incorporate “smart” molecular-scale biological components. These have attracted considerable interest from both the biomedical and biotechnology communities worldwide. However, little has been done to date in developing integrated nanodevices and systems such as microanalytical systems incorporating novel, engineered nanobioconstructs and their analogues for use in integrated nanodevices and systems such as bio-optical hybrid sensors capable of very sensitive and selective nanoscale detection due to enhanced performance characteristics as determined by a prescribed biohybrid Figure of Merit (FoM). Potential applications include microsystem applications requiring low-level light detection capability (e.g. micro total analytical systems (μTAS) for immunoassay, genomics and proteomics), such as “point-of-care” diagnostic medicine, biotechnology, space bioengineering, and countermeasures to biowarfare for defense.

In general, the current state of art for engineering design as taught by Koen (Koen, 1987) and many others (Otto and Wood, 2001), has not led to the achievement of device components, stand-alone devices, nor engineered systems that function or otherwise perform at a prescribed FoM and oftentimes typically perform at levels significantly below optimal FoM levels and theoretically achievable maximum FoM limits.

The well known area of thermoelectric device design exemplifies the present ability of engineering design heuristics to achieve a desirable thermoelectric FoM (i.e., ZT) that significantly exceeds current ZT device values of ˜1 although a ZT value of 4 is theoretically possible (Rowe, D. M., 1995). The present inability by those skilled in the art to achieve desired material and device FoM's is essentially true for virtually all engineering device design applications spanning diverse disciplinary fields and broad industry product segments.

In recent years, less effective and predictive empirical approaches have been used to devise novel hybrid devices that incorporate naturally—derived, or mimetics thereof, biological materials and constructs that have resulted in enhanced device performance relative to their non-hybrid engineered counterpart. To date, however, the engineering method does not teach how to design, select, modify or otherwise alter smart, nanoscale energy-interactive materials (e.g., molecular-scale biophotonic components) derived from natural or biomimetic analog constructs, a priori, in spite of their intrinsically superior and potentially adaptable structural and performance characteristics. Nor does the engineering method show those skilled in this art how such nanoscale materials can be further embodied or employed as components, or as stand-alone devices, that are capable of producing robust and scalable energy-interactive biohybrid devices and systems, a priori, to function at a desired FoM not yet achievable by conventional engineering means.

Photoactive semiconductors such as Si photovoltaic cells (as one example of a large scale device) have long been known. They have been employed in various devices and applications for years. Their varying responsivity to certain light wavelengths throughout the visible spectrum has been observed as well. On the biological side, thermophilic photosynthetic bacteria such as Chloroflexus aurantiacus (C. aurantiacus) and other species have been studied and reported upon. The photosensitive “antenna” cells, “chlorosomes,” of these organisms have been studied and reported upon, as well. Perhaps as a result of inconsistency of results with photosynthetic bacteria, these organisms and their chlorosomes have not been incorporated into useable devices. There has been no successful synthesis of photosensitive semiconductor materials with the chlorosomes of photosynthetic bacteria reported. A need exists for improvement of the performance of photoactive devices throughout the light spectrum, and for techniques for using the good photosensitivity of photosynthetic bacteria in photoactive devices. More fundamentally, there is a need to identify inconsistencies in the photosensitivity (or other photonic or electroactivity) of biological specimens and to apply a method or methods to ameliorate or eliminate such inconsistencies.

As one means of gathering knowledge about a system, Design of Experiment (DOE) analysis is a widely used statistical modeling approach, reported in detail elsewhere (Montgomery, 1991). A unique advantage of DOE, particularly as applied to complex adaptive systems, is its ability to elucidate, not only the effect of the controlling variables, but also their complex interactions. Use of DOE analysis with biological or hybrid biological/nonbiological devices and systems has not been encountered. In particular use of the powerful DOE approach in connection with forced adaptation in biological systems (such as bacteria) to move the systems toward a more consistent (i.e. dependable) performance is not known. Figure of Merit (FoM) is another concept often used in engineering (among other fields such as economics, chemistry, astronomy, etc.). FoM is a measure of a device's performance. It is used in many contexts. However FoM as a design-driving measure, particularly with respect to adaptive biological organisms-based systems, devices and components is considered to be a radical departure from other uses of this concept. Further, as applied to biological organisms, parts thereof or systems made up of such organisms, a means to control multiple environmental variables is needed if the DOE approach is to be applied.

The transfer function of a device, circuit or system is another engineering concept that is well understood. However, that concept has not ordinarily been applied to biological systems, if at all. A need exists to apply engineering concepts like DOE, FoM and the transfer function to the analysis, evaluation and design of biological, bioengineered and hybrid systems, components and devices.

SUMMARY

Broadly, the present invention encompasses equipment and methods for the synthesis, processing, design and manufacturing of high performance, scalable, adaptive and robust energy-interactive hybrid materials, devices and systems combining biological and nonbiological technologies. Specifically, an exemplary embodiment of the invention adapts powerful engineering concepts to the engineering of biological components that are to be used in manufactured devices and systems, including hybrid devices and systems.

FIG. 1 exemplifies a novel method that will guide those skilled in the art to achieve the design and development of high performance hybrid materials and devices. As illustrated, several key steps are depicted in FIG. 1 that show one skilled in the art how to achieve desired and even optimal hybrid device designs that utilize smart, nanoscale constructs acquired, harvested or otherwise derived directly from complex living organisms. A preferred embodiment is the use of a multiple input-multiple output apparatus, such as a multiple input—multiple output environmental chamber (i.e., MIMO/EC), and applicable computational algorithms to extract useful and exploitable hybrid device design heuristics. Use of this method will result in a desired and prescribed Figure of Merit in spite of the use of previously unknown or poorly defined or characterized nanoscale biological constructs and their function. In applied form, the novel engineering design method described herein will provide a means to identify or otherwise exploit intractable, or very difficult to identify, useful engineering specifications. A preferred embodiment of this invention is shown in FIG. 2, an illustration of a novel method and apparatus for the design and development of high performance hybrid materials and devices. One application of the proposed invention is the enhancement of well-known photoactive semiconductor devices, such as Si photovoltaic cells using nanoscale biophotonic constructs that are either acquired, harvested or otherwise manipulated in their natural or adapted state using the method and apparati described herein to achieve desired FoM performance characteristics. Although commercially available Si photovoltaic cells have been employed in various devices and applications for years, their FoMs are typically low despite detailed knowledge of their structure and function and the ability to prescribe device performance specifications from use of selected light wavelengths throughout the visible spectrum, as well as, related device specifications associated with the engineering transfer function.

The transfer function of a component, device, or system is a useful engineering concept, directly related to the FoM, that is well known and understood by those skilled in the art. However, the use of a transfer function and related FoM concepts have not been generally applied and prescribed to biological constructs intended for use in the design of biohybrid devices and systems, if at all. Thus, an unmet and nonobvious need still exists to use well known engineering heuristics such as, the design of experiments (i.e., DOE), FoM and the transfer function for the analysis, design, and evaluation of bioengineered hybrid components, devices and systems.

To demonstrate the novelty and utility in the use of the hybrid device design heuristic to achieve high performance hybrid materials and devices (FIG. 2), the invention described herein improves the device performance (i.e., the FoM) of a stand-alone, commercial silicon photovoltaic device (Si PV) using a nanoscale bioderived construct with generally unknown engineering specifications. However, the methods and apparati taught herein generally apply to the design and exploitation any smart nanoscale or integrative nanoscale material, construct, or system, or mimics thereof, that is amenable to the FoM enhancement of a hybrid device or system.

A typical FoM of a Si PV device is generally less than 1 and typically only ˜0.28-0.32. Although a number of potentially useful hybrid design approaches can be employed to improve the SiPV FoM using the invention taught herein, the use of a nanoscale biophotonic construct having desired complementary energy transfer properties constitutes a potentially viable hybrid design approach. One such nanoscale biophotonic construct having potentially useful and exploitable engineering specifications to enhance the FoM of a photonic device, such as a SiPV device, is the nanoscale pigment-protein supramolecular construct known as a light antenna structure that function as energy funnels in thermophilic photosynthetic bacteria such as Chloroflexus aurantiacus (C. aurantiacus) and other photosynthetic species. These highly quantum efficient photosensitive constructs (also known as “chlorosomes”) are known to perform significant photonic energy shifts (red shift). In the case of the chlorosome associated with the C. aurantiacus, an input photonic energy at a wavelength of ˜460-480 nm is typically shifted to ˜800-820 nm with very little energy loss.

Typically SiPV devices are more sensitive to higher photonic wavelengths and generally most sensitive to the near infrared region (i.e. 800-900 nm) of the electromagnetic spectra. Thus, in principle, the use of biologically-derived light antenna structures, as well as mimics or analogs thereof, could potentially enhance the FoM of a Si PV device if exploitable engineering specification(s), such as the transfer function or its associated FoM, could be identified, acquired, developed and subsequently employed successfully in a SiPV engineered hybrid device or system that meets a prescribed and verifiable FoM that validates the desired performance of the hybrid biophotonic device. However, the achievement of desired FoMs using hybrid device and system approaches is not obvious to those skilled in the art of device design and development and empirical combinations of smart materials or components used in the design and manufacture hybrid device can oftentimes lead to device and system performances (i.e., FoM) inferior to nonhybrid device and system counterparts.

A preferred embodiment of the invention makes use of well-known design algorithms, such as the Design of Experiment (DOE), among many others known and appreciated by those skilled in the art. DOE analysis is a widely used statistical modeling design tool reported in detail elsewhere (Montgomery, 1991). A unique advantage of DOE, particularly as applied to complex adaptive systems, is its ability to elucidate, not only the effect of the controlling or independent variables, but also their oftentimes complex interactions.

The use of DOE analysis in combination with a novel MIMO/EC apparatus can be used to identify, acquire or otherwise produce useful and exploitable engineering hybrid device and system specifications from complex biological constructs in their isolated or natural state or environment, or mimics thereof. In particular, the combined use of DOE with the MIMO/EC apparatus can provide a novel and powerful design heuristic to achieve desired engineering specifications of nanoscale-based constructs via their identification and/or modification from complex, adaptive systems, such as, viable organisms.

The use of the DOE—MIMO/EC apparatus in this embodiment is most useful when it may be desirable to modify one or more properties of a complex, adaptive construct through the ‘forced adaptation’ of a modifiable biological component of a viable, complex systems (such as bacteria). This produces the desired modification of a potentially useful property or characteristic of e.g. a nanoscale-based component that is useful to achieve a desired performance level (i.e. FoM) of a device or system in which that is not otherwise achievable by its nonhybrid.

In one embodiment of the invention by varying the environmental conditions under which a biological component of, for example, a hybrid device, is grown, a transfer function for that component can be altered. Using the MIMO/EC of this invention, a biological component may be force adapted in such a manner as to affect a modification of a transfer function that governs its outputs under given inputs. The desired transfer function can thus be engineered into a biological component, within bounds.

In one exemplary embodiment of the invention, the methods and equipment of the invention are used to engineer an exemplary hybrid photoactive component. That component combines a hitherto acceptable photoactive semiconductor device with a biological mechanism that has extreme high photoactive performance to achieve performance unprecedented in devices of the type. This hybrid device, itself an exemplary and preferred embodiment of one aspect of this invention, uses a constituent of a photosynthetic bacterium to enhance the response of a semiconductor photoactive device across the intended spectrum of its use.

With the methods and equipment of this invention, chlorosomes of the thermophilic green photosynthetic bacterium Chloroflexus aurantiacus (C. aurantiacus) are successfully coupled to a photoactive semiconductor device to derive enhanced performance across the relevant spectrum.

Here, using design of experiment (DOE) methodology, adaptive biological units, such as cells, are force-adapted to achieve consistent performance in the characteristics of interest. In this, a multiple input—multiple output environment chamber (the above-mentioned MIMO/EC) affords the ability to force adapt the bacteria from which these chlorosomes are gathered.

This embodiment is focused on exploiting biosystems at the nanoscale for their utility as functional ‘device’ components in a proposed biohybrid microdevice. More specifically, a design feasibility study was implemented to evaluate the efficacy of a naturally occurring nanoscale biophotonic, light adaptive ‘antenna’ structure (the chlorosome), isolated from C. aurantiacus. The overall objective was to assess its utility as functional device component that would enhance the spectral performance characteristics of well-characterized photonic devices, i.e., solid-state photovoltaic.

The chlorosomes are nanoscale, optical functional units (100×30×10 nm). They can transfer photonic energy at high quantum efficiencies (69-92%) and ultra-fast rates (picoseconds). They were fabricated into programmed arrays on solid substrates and fully characterized. These biological assemblies were subsequently integrated with the well-characterized photodetectors and evaluated for their potential to selectively enhance performance the spectral regions where the photodetectors are inherently insensitive.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 is a conceptual block diagram illustrating elements in the design and development of a device, in particular a hybrid device of biological and nonbiological content;

FIG. 2 is a conceptual flow chart of the design process for designing a hybrid device using a multiple input, multiple output environmental chamber and employing a figure of merit to gauge the performance of a biological component;

FIG. 3. is an image of C. aurantiacus by a scanning electron microscope;

FIG. 4 is a cartoon schematic rendering of chlorosomes of C. aurantiacus in place in a cytoplasmic membrane;

FIG. 5 is a diagrammatic (cartoon) illustration of a chlorosome of the bacterium C. aurantiacus with its four major subunits (the chlorosome designated herein the RC⁺ chlorosome);

FIG. 6 is a diagrammatic (cartoon) illustration of the chlorosome of the bacterium C. aurantiacus of FIG. 5, but with two of its four subunits, the B808/866 protein light harvesting apparati and a reaction center removed (the chlorosome thus modified designated herein the RC⁻ chlorosome);

FIG. 7 is a diagrammatic (cartoon) illustration of the chlorosome of FIG. 6 with parts broken away for clarity showing contained rod-like structures of Bchl c;

FIG. 8 is a functional block diagram in the form of a flow chart of optical interactions of the components of the chlorosome shown in FIG. 5;

FIG. 9 is a plot of absorbent spectra data for a C. aurantiacus chlorosome;

FIG. 9A is a normalized absorbance spectra plot of the RC⁻ chlorosome;

FIG. 10 is a diagrammatic block diagram in the form of a flow chart indicating the optical interaction of the parts of the chlorosome of FIG. 6;

FIG. 11 is a diagrammatic illustration, partly in section, of a hybrid photovoltaic device in accordance with the invention;

FIG. 12 is an enlarged fragmentary cross-sectional view along the line 12-12 of FIG. 11 and shows the chlorosomes like that of FIG. 6 adherent to a transparent plate;

FIG. 13 a is a normal percentage probability plot and FIG. 13 b is the interaction plot between temperature and percent volume for a design of experiments analysis where the output variable to be studied was the ratio R1 of absorbance at 740 nm to absorbance at 808 nm;

FIG. 14 a is the normal percent probability plot and FIG. 14 b the interaction plot between temperature and percent volume media to air for a design of experiments analysis where the output variable studied is the ratio R2 of absorbance at 740 nm to absorbance at 366 nm;

FIG. 15 a is a plot of three replicates of a full spectra of C. aurantiacus at one dilution and FIG. 15 b plots full spectra of absorbance of C. aurantiacus at multiple concentrations;

FIG. 16 a is a plot of correlation between absorbance and cell count at 650 nm wavelength for C. aurantiacus and FIG. 16 b is a plot of correlation between absorbance and cell count at 740 nm wavelength;

FIG. 17 is a plot of correlation between absorbance and number of RC⁻ chlorosomes of C. aurantiacus at 650 nm wavelength and a zoomed-in-plot of the first four data points in that correlation showing close linearity between the two variables;

FIG. 18 is a plot of absorbance and emissions spectra of chlorosomes of C. aurantiacus;

FIG. 19 is a plot of percent enhancement of a SiPV for percent coverage by chlorosomes of C. aurantiacus;

FIG. 20 is a functional block diagram that illustrates the use of a figure of merit in the development of a biological hybrid device with feedback from the Figure of Merit determination through a DOE or the like development program and feedback from the device performance;

FIG. 21 is in FIG. 21( a) a formula for the photonic figure of merit devised for C. aurantiacus, in FIG. 21( b) a tabulation of the measures going into that formula for seven specimens and in FIG. 21( c) a block diagram illustrating the interaction of the major contributing factors to the figure of merit;

FIG. 22 is a diagrammatic illustration of a multiple input, multiple output environmental chamber having nine individual compartments;

FIG. 23 a perspective view of an environmental chamber like that diagrammatically illustrated in FIG. 22;

FIG. 24 is a schematic illustration of a generalized hybrid device in accordance with the invention;

FIG. 25 is a schematic illustration of a further generalized hybrid device in accordance with the invention;

FIG. 26 is still another schematic illustration of another generalized hybrid device in accordance with the invention;

FIG. 27 is a schematic, generalized, enlarged, fragmentary cross-section view of a biological component such as those employed in the devices of FIGS. 24-26 and shows fluorescent units deployed in a matrix; and

FIG. 28 is a further schematic, generalized, enlarged cross-section view of a biological component such as those employed in the devices of FIGS. 24-26, and shows fluorescent units adherent to the surface of a nonbiological photoactive element.

DETAILED DESCRIPTION

FIG. 24 is a schematic view of a generalized hybrid device 200. The device 200 has a biological component 201 and a nonbiological component 202. In broadest terms the biological component is located in energy transmitting relation to the nonbiological component 202. Activated, the biological component transmits energy to the nonbiological component causing a change in state (such as an electrical characteristic change) of the nonbiological component. An energy transmitting intermediate layer 203 may separate the components 202 and 203 or the components may be in contact or spaced apart. The biological component may be made up of units of active biological material or materials (or analogs of such material or materials if available). And the units may be units harvested from a larger organism or organisms. The biological component 201 may be comprised of a matrix containing the units. In the particular embodiments described here the biological component 201 fluoresces, exhibiting what is termed a Stokes shift, the emission of light at a wavelength or wavelengths different from light illuminating and activating the biological component 201. In the exemplary embodiments described here the nonbiological component is photoactive, that is to say photovoltaic, photoconductive or photoemissive. The material of component 202 is typically a photoactive semiconductor. Light emitted from the biological component illuminating the photoactive nonbiological component causes the photoactive nonbiological component to exhibit its characteristic photoactive behavior, i.e. developing a voltage, exhibiting a change in resistance or emitting electrons.

Conceivably the energy communicated from the biological component 201 to the nonbiological component 202 could be transmitted by other than light. The transmission could be by the mechanism known as FRET (fluorescence resonant energy transfer) or by another mechanism whereby the nonbiological component 202 is caused to react sympathetically to the action of the activated biological component.

In the case of fluorescing biological component 201 and the photoelectric nonbiological component 202, the intermediate layer 203 could be a clear plastic or glass. Alternatively the component 201 may be in contact with the component 202 or they may be spaced apart by for example an air gap, a vacuum or a clear gas. If the biological component 201 includes a matrix, that could be a clear plastic employed to support active biological units or a liquid such as water or a solvent supporting such units in a suspension such as a colloidal suspension.

Clearly what is important in these exemplary photoactive devices is that the components 201 and 202 be arranged in light communicating relation with the component 202 located to receive light emitted by the component 201. Also, when illuminated with light in one region of the spectrum at 204, the component 201 emissions at 205 are to be in another, different region of the spectrum to which the component 202 is responsive (or has heightened responsivity as compared to other regions of the spectrum to which the component 202 is not responsive or has lower responsivity). That is to say, the characteristic photoactivity of the component 202 results from (or is enhanced by) being illuminated by light in that region of the spectrum in which the component 201 emits light. Table A identifies exemplary biological organisms and the photoactive materials therein capable of being used to form the component 201. Table B gives the wavelengths of absorbed and emitted light of the photoactive materials identified in Table A either in an appropriate solvent, in the case of the chlorophylls, or standing alone in the cases of the carotenoids and bilins. Table C lists photosensitive nonbiological or engineered elements capable of being used as the component 202. Table D lists pairings of Table A and Table B elements with optical and photoactive qualities qualifying them for use together in the device of FIG. 24. The resultant device of FIG. 24 is one that exhibits the characteristic photoactivity of component 202 when illuminated with light in the spectral region to which the component 202 is insensitive or has less than desired sensitivity.

TABLE A Photosynthetic Pigments and Pigment Proteins Utilized in Light Antenna Structures from Various Biological Organisms* B B B Chl B Organism Chl a Chl b Chl c Chl d Chl a Chl b c, d, e Chl g Carotenoids Bilins Purple + + + Bacteria Green Sulphur + + + Bacteria Green + + + Nonsulphur Bacteria Heliobacteria + + Cyanobacteria + + + + + + Green Algae + + + Diatoms + + + Brown Algae + + + Dinoflagellates + + + Cryptomonads + + + + Red Algae + + + + Plants (e.g. + + + maize) Chl a = Chlorophyll a; Chl b = Chlorophyll b; Chl c = Chlorophyll c; Chl ad = Chlorophyll d B Chl a = Bacterial Chlorophyll a; B Chl b = Bacterial Chlorophyll b; B Chl c = Bacterial Chlorophyll c; B Chl d = Bacterial Chlorophyll d. *Ref: Molecular Mechanisms of Photosynthesis, R. Blankenship, Blackwell Scientific, 2002.

TABLE B Spectroscopic Properties of Chlorophylls and Bacteriochlorophylls in Vitro and of Pigments* Absorption (max) Pigment Solvent [nm] Emission† Chl a diethyl 662, 578, 430 670 ether Chl b diethyl 644, 549, 455 652 ether Chl c pyridine 640, 593, 462 648 Chl d diethyl 688, 447 696 ether B Chl a diethyl 773, 577, 358 781 ether B Chl b aceone 791, 592, 372 800 B Chl c diethyl 659, 429 667 ether B Chl d diethyl 651, 423 659 ether B Chl e acetone 649, 462 657 B Chl g acetone 762, 770 566, 405, 365 Carotenoids 400-500 550-675 (Range) Bilins (Range) 550-650 + {close oversize brace} 560-660 Biliproteins (Range) 540-660 *Ref: Molecular Mechanisms of Photosynthesis, R. Blankenship, Blackwell Scientific, 2002. †Estimated.

TABLE C Materials Used for Photovoltaics (PVs) Eff* Engineered (%) Material approx Use* Spectral Sensitivity** Inorganic PV Materials* Amorphous Silicon 10 Most Common Form of Thin UV & IR Low; Highest (550-650) Film PV Cadmium Telluride 17 Sig & Growing Share of Thin UV Low; Highest @ IR ~900 nm [CdTE] Film PV Market Cu—In—Ga—Se 20 Growing Share of Thin Film UV Low; Highest 600-800 nm Market Poly(micro) 20 Widely USed Highest @~500-600 nm Crystalline Silicon Monocrystalline 25 Most Common PV Material in UV Low; Vis Mod; High (750-1000) Silicon Use Today Indium Phosphide 22 In Lab R&D Only UV & IR Low; Highest @600-800 nm Gallium Arsenide 25 Not Widely Used Yet UV Low; Highest @ 900-1200 nm (GaAs) GaAs/InP/Ge Hybrid 35 Lab R&D Only Organic PV Materials # Doped Pentacene 4 to 8 Currenlty Under Peak QE Range: 400-650 Commercialization Homojunction 2.4 36% @ 650 nm spiro-OMeTAD† 2.56 Dye-Sensitized Solar Cell 38% @ 520 nm Technology MDMO-PPV-PCBM†† 2.5 50% @ 470 nm Cu 3.6 18% @ 620 nm phthalocyanine/C60 *Efficiencies from cited reference: ‘Positioning-Thin Film Photovoltaics for Success’ A Nanomarkets White Paper March 2008; www.nanomarkets.net **Estimated # From ref: Organic Photovoltaic Films, Materials Today, May 2002, p23 †2,20 7,70-tetrakis(N,N-di-p-methoxyphenyl-amine)-9,90-spirobifluorene (spiro-MeOTAD) ††MDMO-PPV (poly)[2-methyl,5-(3*,7** dimethyl octyloxy)]-p-phenylene vinylene): PCBM ([6,6]-phenyl C61 butyric acid methyl ester)

TABLE D Biohybrid Combinations of Engineered PV Components and Bioderived Light Antenna Components Relative Biophotonic Exemplary Biophotonic Pairing Engineered Output Biohybrid Abs Bioderived Emiss Engineered Abs Rel to Example nm Component B [max] Component A [nm] Eng Mat 1 470/740/750 Chlorosomes 772/805/808 Monocrystalline 750-1000 Efficiency (from different silicon >25% species) 2 670 LHCI 730 Monocrystalline 750-1000 Efficiency silicon >25% 3 470/670/740 Chlorosome + LHCI 730 + 808 Monocrystalline 750-1000 Efficiency silicon >25% 4 FMO Protein (BChl 785 Monocrystalline 750-1000 Efficiency a) silicon >25% 4 See below Phycobilisomes 537-660 Amorphous 550-650 Efficiency (all) Silicon >10% (alone or in comb) 4a 565 (495) R-Phycoerythrin 575 Amorphous 550-650 Efficiency Silicon >10% 4b 545 B-Phycoerythrin 575 Amorphous 550-650 Efficiency Silicon >10% 4c ~495 (545)  Y-Phycoerythrin ~563 Amorphous 550-650 Efficiency Silicon >10% 4d 615 C-Phycocyanin 647 Amorphous 550-650 Efficiency Silicon >10% 4e 617 (555) R-Phycocyanin 637 Amorphous 550-650 Efficiency Silicon >10% 4f 652 Allophycocyanin 660 Amorphous 550-650 Efficiency Silicon >10% 4g 566 Phycoerythrin 566 617 Amorphous 550-650 Efficiency Silicon >10% 4h 575 Phycoerythrocyanin 625 Amorphous 550-650 Efficiency Silicon >10% 5 375/800/850 LH2 ~800 Indium 600-800 Efficiency Phosphide >22% 6 470/740/750 Chlorosomes 772/805/808 Indium 600-800 Efficiency Phosphide >22% 7 773, 577, 358 FMO Protein 785 Cu—In—Ga—Se 600-800 Efficiency >20% 8 644, 549, 455 Chl b 648 Doped 650 Efficiency Pentacene >2.4% Homojunc Note: utilizing methods taught in specification, biophotonic structures can be engineered to acquire different biophotonic and other desired properties, e.g, absorbance and emission values, to obtain desired figures of merit that exceed engineered materials alone when put into exemplary pairings such as depicted in above biohybrid examples

Table E relates species, light antenna structure, pigment and/or pigment protein with wavelengths of absorbed light and emitted light.

TABLE E Biophotonic Light Antenna Structures and Light Absorption Emission Values Pigment or Absorption Light Antenna Pigment-Protein (Max) Emission† Structure Complex [nm] [nm] Specie PERIPHERAL MEMBRANE Phycobilisomes Bilins 550-650 Cyanobacteria/Red [Bilins + phycocyanobilin Algae Biliproteins] [Bilins + phycoerythrobilin Biliproteins] [Bilins + Biliproteins 540-660 560-660 Biliproteins] [Bilins + R-Phycoerythrin 565 (495) 575 Red Algae Biliproteins] [Bilins + B-Phycoerythrin 545 575 Cyanobacteria/Red Biliproteins] Algae [Bilins + Y-Phycoerythrin ~495 (545)  ~563 Cyanobacteria Biliproteins] [Bilins + C-Phycocyanin 615 647 Cyanobacteria/Red Biliproteins] Algae [Bilins + R-Phycocyanin 617 (555) 637 Biliproteins] [Bilins + Allophycocyanin 652 660 Cyanobacteria/Red Biliproteins] Algae [Bilins + Phycoerythrin 566 566 617 Biliproteins] [Bilins + Phycoerythrocyanin 575 625 Green Sulphur Bacteria Biliproteins] Chlorosome B chl c, d, e 659, 429/651, 423/ 808 Chloflexus Aurantiacus 649, 462 Chlorosome BChl c, a, Carot, Quin 470/750 772 & 805 Chlorobium tepidum FMO Protein B Chl a 773, 577, 358 785 Fucus Serratus LH2 Complex B Chl a, Carotenoid 850, 800, 750-470 890 Rhodopseudomonas Acidophila Green Sulphur Bacteria Peridinin- B Chl a & Carotenoid 660, 535, 437, 350 679, 675 Dinoflagellates Chlorophyll Protein (PCP) LH2 Complex BChl/Carot/prot 800-850 (375)   ~860 Purple Bacteria subunits INTEGRAL MEMBRANE Fused PS1 RC chlorophyll; β-carotene 430-660 ~700 Plants, Algae, Complexes Cyanobacteria Green Sulp Bact Bchl a; carotenes 450-850 ~860 Green Sulphur Bacteria RC Complex Heliobact Bact Bchl a; carotenes 450-850 ~860 Heliobacterial RC Complex Core CP43 & CP47 Chl a & œ-Carotene 660, 450, 430 ~700 Plants, Algae, Complex PS2 Cyanobacteria LH1 Complex B Chl a & œ-Carotene 875 (380) ~860 Anoxygenic Bacteria Accessory LHCI Complexes Chl a & Chl b 676, 470 715-735 Plant (Maize) of PS1 LHCII of PS 2 Chl a & Chl b 675, 650 679 Plants & Algae LH2 Complex  832 (~375) 800 Purple Bacteria PS 2 Chl a PSI & PSII 450 710 Algae (Diatom- PTricornutum) †estimates FMO = Fenna-Mathews-Olson LH1 = Light Harvesting 1 Complex; LH2 = Light Harvesting 2 Complex' PSI = Photosystem I; PSII = Photosytem II LHCI = Light Harvesting Complex I; LHCII = Light Harvesting Complex II; Bacterial RC = Bacterial Reaction Center *References: Molecular Mechanisms of Photosynthesis, R Blankenship, Blackwell Scientific, 2002. Photosynthesis (Third Edition), David W Lawlor, BIOS Scientific Publishers, 2001. Probing Photosynthesis. Mechanisms Regulation & Adaptation, M Yanus, U Parthre, O Mohanty Eds), Taylor & Fancis, 2000. Chlorophylls and Bacteriophylls, B Grimm, RJ Porra, W Rudiger, H Scheer, Eds., Springer, 2006.

In each of the organisms identified in Table A, the photoactive protein or protein pigment is present in an antenna structure. The methodology for isolating the unit that forms part of that antenna structure contains the identified photoactive pigment or pigment protein is well documented in the literature. One or more of the publications listed below under Isolation, Separation and Harvesting Techniques for Light Antennas and Subunits sets forth a technique for isolating one or more of the fluorescent units identified as useful in practicing an embodiment of the invention. Each publication listed is incorporated herein by reference. Typically isolating the chosen structure involves first isolating the antenna structure and then removing from that antenna structure a reaction center (RC) that ordinarily absorbs light at wavelengths emitted by the photoactive material identified in Table A to produce protons. Other structure that does not contribute to the Stokes shift phenomenon may be removed and discarded as well.

The antenna structures that contain the photoactive pigments and pigment proteins used in the component 201 are nanoscale structures of various shapes and may be directional in their light absorbing and emitting abilities, absorbing at one end and emitting at the other, say. In which case a majority to a totality of these structures are preferably similarly oriented within the component 201 so that the component 201 is similarly directional in its light absorbing and emitting characteristic.

Orienting the units can be accomplished in a number of ways. In the specific example that follows, because the chlorosomes of that example, when stripped of their reaction centers, have a hydrophilic portion or base plate at the light emitting end, a hydrophilic glass slide was used to support the chlorosomes, causing the hydrophilic portion of the chlorosomes to come to rest on the slide. Other techniques for manipulating nanoparticles such that their orientation can be assured are addressed in the literature. See, for example, Lavan, D. et al., “Approaches for biological and biomimetic energy conversion,” 5251-55, PNAS, vol. 103, no. 14, 2006; Morris, C. et al., “Self-Assembly for Microscale and Nanoscale Packaging Steps Toward Self-Packaging,” 601-11, IEEE Transactions on Advanced Packaging, vol. 28, No. 4, 2005; Yoshino, M. et al., Engineering surface and development of a new DNA micro array chip,” 274-86, Wear 260, 2006; Kane, R. et al., “Patterning proteins and cells using soft lithography,” Biomaterials 20, 2363-76, 1999. Each incorporated herein by reference.

It should be recognized that the structure of the device 200 of FIG. 24 may be repeated many times throughout a bigger photoelectric device. The device 200 or bigger photoelectric device may be encapsulated in such a way as to permit the biological component 201 to be illuminated. For example by encapsulation in a clear plastic or glass.

FIG. 25 is a schematic view of a further hybrid photoelectric device 210. A nonbiological component 212 and a biological component 215 have the structure and characteristics of the nonbiological and biological components 202 and 201 of the device 200 of FIG. 24. In a preferred photoactive embodiment the nonbiological photoactive component 212 receives the full spectrum of illuminating light at 213. The biological component 215 receives the full spectrum of illuminating light at 216 and emits light at 217. The nonbiological photoactive component is responsive to (or has heightened responsivity to) light in a range of wavelengths within the full spectrum of the illuminating light at 213 as compared to other wavelengths, but is comparatively nonresponsive to (or less responsive to) light in another range of wavelengths within the full spectrum of the illuminating light at 213. The biological component 215 is responsive to light within the range of wavelengths to which the nonbiological component 212 is nonresponsive (or has low responsivity) and illuminates the nonbiological component with light at 217 that is at wavelengths among the wavelengths to which the nonbiological component is responsive (or has comparatively higher responsivity). As a result the overall device of FIG. 25 acts as a photoelectric device responsive to a wider spectral range than a device employing just the nonbiological component 212 as the sole photoactive element. Again the biological and nonbiological components may be chosen and assembled in the same manner as in the device 200 of FIG. 24. Also multiple biological units of differing characteristics may be employed in combination with the nonbiological component 212 to employ even more regions of the spectrum to which the nonbiological element 212 is not responsive or is less responsive.

As with the device of FIG. 24, the device 210 of FIG. 25 may be repeated many times throughout a larger overall device. The device 210 or larger overall device may be encapsulated so long as the biological component or components can be illuminated. Also, as is true of the device 200 of FIG. 24, because of the minute nature of the biological antenna structures used in the preferred embodiments, the device can itself be nanosized or by use of many multiples of the biological antenna structures can be much larger as fits a particular use.

A further embodiment of the invention is schematically illustrated in FIG. 26. The biological and nonbiological components of this device may be as described with respect to the biological and nonbiological components of the devices 200 and 210 of FIGS. 24 and 25. In a preferred photoactive embodiment a device 220 includes a nonbiological or engineered photoactive component 222, a first photoactive biological component 223 is responsive to light in a first region of the spectrum at 224 to emit light at 225 in a second region of the spectrum different from the first region of the spectrum by which it is illuminated. The second region of the spectrum includes wavelengths at which the nonbiological component 222 responds photoactively or has heightened photoactive responsivity as compared to other wavelengths. A second photoactive biological component 227 is of a photoactive biological material different from the photoactive biological material of the component 223.

In a first embodiment of the device 220, the component 227 is responsive to light in a third region of the spectrum at 228 to emit light at 229 in a region of the spectrum different from the third region of the spectrum by which it is illuminated. The light emitted by the second biological component 227 is in a region of the spectrum at which the nonbiological component is photoactively responsive or has heightened photoactive responsivity. That may be the same as the second region of the spectrum at which light is emitted by the biological component 223, or the light emitted by the second biological component 227 may be in a region of the spectrum overlapping or different from the spectrum of the light emanating from the first biological component 223. In this embodiment of the device 220, then, the device is photoactive, or has improved photoactivity, in the manner of the photoactive nonbiological component 222, but at several regions of the spectrum at which the component 222 is not photoactively responsive or has lower than desired photoactive responsivity.

In a second embodiment of the device 220 of FIG. 26, the two photoactive biological components 223 and 227 may be active in the same or overlapping regions of the spectrum in the light illuminating them at 224 and 228, but may emit light in differing regions of the spectrum at which the nonbiological component 222 is photoactive comparatively more highly photoactive to take advantage of a photoactivity profile of the material of the component 222 that has several peaks at several different wavelengths, that is has heightened responsivity at various different wavelengths.

Additional biological components of characteristics different from the component 223 and 227 may also be employed with the nonbiological component 222 so as to extend the range of wavelengths impinged on the biological components that activate the photoelectric characteristic of the nonbiological component 222 or to provide further illumination of the photoelectric element 222 in further regions of the spectrum where the particular photoelectric material has heightened responsivity. Again encasement of the device 220 is possible as long as the biological components can be illuminated. This device too can be realized on the nanoscale or can be scaled up by the use of many of the active elements making up the biological components 223 and 227. Also, as with the devices 200 and 210 of the FIGS. 24 and 25, multiple devices 220 may be incorporated in a much larger overall device.

In each of the embodiments of FIGS. 24, 25 and 26 the photoactive nonbiological component may be a photovoltaic cell in which case a voltage V is developed across the output electrode pairs 206 and 207, 218 and 219, and 230 and 231 in response to illumination of the nonbiological component by light in the proper range of wavelengths. The fluorescent units making up the biological components 201, 215, 223 and 227 of FIGS. 24-26 can be a mixture of such units derived from more than one of the organisms of Table A (and such other organisms as may be identified).

In addition to the photovoltaic materials listed, the nonbiological component may be photoconductive materials or photoemissive materials. Photoconductive materials are materials that exhibit decreased electrical resistance when exposed to infrared rays, visible light or ultraviolet light. Known photoconductive materials include cadmium selenide, cadmium sulfide, germanium, lead sulfide, selenium, silicon and thallous sulfide. Photoemissive materials are substances that emit electrons when exposed to infrared, visible light or ultraviolet radiation. Known photoemissive materials are cesium, potassium, rubidium and sodium. As in the case of choosing photovoltaic material for a device as discussed, photoconductive materials or photoemissive materials are chosen so that they are responsive (or have heightened responsivity) to illumination by light in the region of the spectrum emanating from the associated photoactive biological component.

FIG. 27 shows, in cross-section, a fragment of a biological component 201, 215, 223 or 227 of FIG. 24, 25 or 26. Fluorescent units 250 are illustrated in a generalized, schematic fashion. The majority have their light emitting ends, which are dotted, facing right toward the photoactive nonbiological member (not shown) and their light absorbent ends, undotted, facing left to be illuminated. The units 250 are suspended in a matrix 251 as discussed above. The units 250 are structures removed from light harvesting antenna structures and contain one or more of the pigments and pigment proteins identified in Table A.

FIG. 28 shows, in cross-section, a fragment of an alternate biological component 201, 215, 223 or 227 of FIG. 24, 25 or 26 adherent to a nonbiological photoactive component 201, 212 or 222 of FIG. 24, 25 or 26. Fluorescent units 250, as described with respect to FIG. 27, are again illustrated in a generalized, schematic fashion. Again the majority have their dotted light emitting ends facing right to the nonbiological photoactive element and their light absorbent ends, undotted, facing left to be illuminated. The units 250 are affixed to a surface of the nonbiological photoactive element adhesively or by linking units known in the art as linkers, either represented in FIG. 28 at 252.

The Table A listing of photoactive biological materials is not necessarily exhaustive. Suitable photoactive biological materials other than those listed may be known or may be developed from species not yet known.

EXAMPLE

The following example sets forth the steps performed in successfully constructing a preferred embodiment of the invention in which the biological fluorescent component uses the light antenna structure (chlorosome) of the bacterium Chloroflexus aurantiacus (C. aurantiacus). The detailed description of this one example will serve those skilled in the art as a blueprint for the preparation of devices of the kind described with respect to FIGS. 24, 25 and 26 using the further biological and nonbiological component discussed above.

The bacteria, Chloroflexus aurantiacus (C. aurantiacus), strain J-10-f1, has the American Type Culture Collection (ATCC) designation number 29366, having been deposited in July, 1976. The ATCC is located at 10801 University Boulevard, Manassas, Va. 20110-2209 U.S.A. The C. aurantiacus bacteria is a green, nonsulfur, flexing/gliding, photosynthetic bacteria. It is thermophilic and can be found in hot springs up to temperatures of 70° C. in large mat-like layers. The layers, when concentrated enough, have an orange coloration.

A freeze fracture image of C. aurantiacus by scanning electron microscopy (SEM) was taken and is reproduced in FIG. 3. In the image small ovals can be resolved. These are the cell's chlorosomes. At this size scale reduction would require specialized EM or other imaging techniques. Thus far, no high resolution structural information has been successfully obtained on individual chlorosome structures, and as such a cartoon schematic representation of the chlorosomes 100 in situ is presented in FIG. 4. There, the chlorosomes 100 are depicted in place in a cytoplasmic membrane 95. A proposed model of a single chlorosome 100 is shown enlarged in FIG. 5 in a 3-D cartoon. From the work of Blankenship, et al., the chlorosome 100 is comprised of four major sub-units: a Bchl c portion 101, a Bchl baseplate 102, B808/866 protein, supra molecular light harvesting complex or apparati 103, and a reaction center (RC) 104.

A chlorosome 110 of the C. aurantiacus bacterium is depicted in FIG. 6. It includes two major supra-molecular pigment-protein subunits. These are the bacteriochlorophyll (Bchl) c 101, and the supra-molecular baseplate complex 102. In its form shown in Fig. the C. aurantiacus chlorosome 100 is here designated RC⁺ (meaning with its RC 104 and B808/866 light harvesting apparati 103 in place). As depicted in FIG. 6 at 110, stripped of its associated reaction center and B808/866 supra-molecular complex 103, the chlorosome of C. aurantiacus is designated RC⁻ (meaning without the RC 104 and B808/866 light harvesting apparati 103). Each sub-unit of the chlorosome 100 illustrated in FIG. 5 is composed of a large number of wavelength-specific light absorbing and transducing molecules.

The first sub-unit involved in light transduction is a lipid sack 101 containing bacteriochlorophyll (Bchl) c, which is organized in units of approximately 10,000 molecules that form rod-like structures 115 (FIG. 7). As represented in the flow chart of FIG. 8 at 115, these molecules transduce photonic energy associated with 740 to 750 nm light in approximately 16 ps with very little loss. Photonic energy at 750 nm is then transduced at 117 by the membrane of the baseplate 102, which is comprised of approximately 500 molecules of Bchl a, to 795 nm to 800/810 nm in 41-175 ps. The B808/866 complex 103 contains 10-20 Bchl a molecules, which absorb at 119 at 808 and 866 nm and transfer at 883 nm in approximately 250 ps. Finally, the last stage is where, at 121, a special pair of Bchl a molecules of the reaction center (RC) 104, convert the light energy into chemical (photochemistry) to emit photons.

FIG. 9 plots absorbance spectra data of isolated chlorosomes of C. aurantiacus noting peaks of interest. There, an absorbance peak at 740-750 nm attributable to the Bchl c rods 113 appears. A peak at 795 nm associated with the Bchl a baseplate is shown. In addition absorption of light in the blue region by the cartenoids is evident and blue secondary absorbance peaks from the Bchl c and a (designated as Soret peaks) occur. A peak attributable to the monomeric form of Bchl c (like its Soret) has a different absorbance wavelength peak than the oligomeric form that comprises the rods 113 in the chlorosomes. Like the Bchl a baseplate peak, the Bchl c oligomeric c peak is in the near infrared (NIR).

Isolated RC⁻ chlorosomes in Tris buffer exhibit the absorbance peaks (solid line) shown in the normalized absorbance spectral plot of FIG. 9A. Immobilizing the RC⁻ chlorosomes in PVAC polymer, however, destroyed the chlorosomes as evidenced by the dashed line normalized absorbance spectrum plotted in FIG. 9A. This was true of other immobilization attempts with other polymers.

Intact C. aurantiacus bacteria display a unique adaptive ability to reversibly and enzymatically assemble and disassemble the foregoing structures to protect the organism from photo-induced damage. As is expected, the spectral peaks of FIG. 9 are highly related to growth conditions of the whole cell C. aurantiacus bacteria. These are also related to the isolation techniques that result in purified chlorosomes. An abbreviated form of the important basic mechanisms of energy transfer that occur between the molecules of the RC⁻ chlorosome are as depicted in FIG. 10.

The carotenoids have been shown to also transfer energy to the Bchl c oligomeric rods as is true of the Soret band (a strong absorbance of a chlorophyll in the blue region of light). However, there are subtle differences in the Bchl c found in the chlorosomes. The Bchl c found in C. aurantiacus chlorosomes are self-assembled (from monomeric form) into oligomeric rods. This results in a shift of the normal Soret (and Q_(y)) band into a redder form. The Bchl a found in the baseplate also has a Soret region in its photonic (blue) spectra. Carotenoids can begin to quench the structures and should be closely watched, as this would cause the device of this invention to operate at lower efficiencies.

In the exemplary embodiment of the invention that was successfully made and tested, the RC⁻ chlorosomes were suspended in a liquid which was then applied to the hydrophobic surface of a borosilicate glass plate 118 as shown in FIGS. 11 and 12. It is the bases 102 of the chlorosomes 110 that adhere to the surface of the plate 118.

As shown in FIG. 11 the plate 118 is supported just above the surface of a glass slide or substrate 120. An epoxy seal 121 is formed about the edges of the plate 118. On or closely spaced above the plate 118 a commercially available silicon photovoltaic cell is supported. Illumination of the chlorosomes and the photovoltaic cell 125 by an LED 127 produces a voltage across the output of the photovoltaic cell 125 as can be observed by a multimeter 129.

As shown in FIG. 18, when excited with 430 nm, 460 nm and 470 nm, which is exactly where the silicon photovoltaic cells is less sensitive, the RC⁻ chlorosome emits at about 810 nm where the silicon photovoltaic cell is sensitive. There is, therefore, a spectral enhancement by the addition of the biological component that is similar to that shown generally in FIG. 18.

In an exemplary laboratory prototype of the device, the SiPV was an Edmond Optics NT53-371 photovoltaic cell. Slide 120 was a Fischer microwells slide, part number 12-568-20 and the plate 118 was a Fischer cover glass, part number 12-541A.

The microslide employed allowed for relatively straightforward application of the chlorosomes. This particular slide has two frosted rings on its surface, one of which is indicated at 131 in FIG. 11. The frosted ring was just sufficiently high above the surface of the slide 120 that a drop of the liquid suspension containing the chlorosomes was retained. The cover glass 118 was rested on the ring 131 and when the suspending liquid had evaporated leaving the chlorosomes adherent to the hydrophobic borosilicate cover glass surface as shown, the epoxy seal 121 was applied. The microwells slide was useful in another respect. Having two of the frosted rings 131, it permitted for the side-by-side construction as illustrated in FIG. 11 and a control. The control could be an identical silicon photovoltaic cell illuminated through the slide 120 and a further glass 118 but absent the chlorosomes, or the control could be as illustrated in FIG. 11 but having the RC⁺ chlorosomes entrapped.

In the arrangement of FIG. 11, the RC⁻ chlorosomes and the light receiving surface of the photovoltaic cells were no more than a millimeter apart. As indicated in FIG. 11, the construction of the off-the-shelf photovoltaic cell placed the light receiving surface 133 of the silicon semiconductor in a metal housing or can 135 to be exposed through a glass closure 137.

Characteristics of the biological component of the hybrid device of FIG. 11 are set forth in Table 1.

TABLE 1 Schematic Pictures of chlorosomes + Si PV Chlorosome Characteristics Size: 100 × 30 × 10 nm Approx Rh: 33 nm (calculated) 41 nm (DLS) Energy Transfer: Strokes Shift: 470-800/810 nm Δλ: 320 nm QE: 69-92% QE Delay Time: 50 ps−1 ns Orientation Yes Control: Number of 4 × 10⁷-8 × 10⁹ chlorosomes particles: Number of 4 × 10¹⁵-810¹⁷ molecules:

Materials and Methods

First, the biological component (the RC chlorosomes), as well as controls, had to be isolated or purchased. Next, several types of characterization had to be performed (and developed in some cases) so that the device fabrication could be accomplished. These involved many steps (and iterations) until sufficient materials were readily available (in the correct form) for use in the hybrid device configuration.

C. aurantiacus cells were grown in ‘D’ media, under 6000 lux 50° C. in a one liter bottle (FIG. 3.2). The ‘D’ mixture is as follows (all chemicals from Sigma): A mixture of 50.0 ml of the medium D stock is added to distilled water with 2.0 gm Difco Yeast Extract, 1.0 gm Glycylgylcine (freebase) adjusting the pH to 8.2. This mixture is then autoclaved for 0.5 hr at 450° C. The medium D stock is prepared by mixing 40.0 ml of Nitch's Solution to 80.0 ml of the FeCl₃ solution in 3.5 l of distilled water with the following traces: 8.0 gm Nitrilotriacetic acid, 4.8 gm of CaSO₄.2H₂O, 8.0 gm MgSO₄.7H₂O, 0.64 gm NaCl, 8.24 gm KNO₃, 55.12 gm NaNO₃, and 8.88 gm Na₂HPO₄. The Nitch's solution is made by placing 0.5 ml concentrated H₂SO₄, 2.28 gm MnSO₄.H₂O, 0.5 gm ZnSO₄.7H₂O, 0.50 gm H₃BO₃, 0.025 gm CuSO₄.7H₂O, 0.025 gm Na₂MoO₄.2H₂O, and 0.045 gm CoCl₂.6H₂O into 1 liter of distilled water. This should be stored refrigerated. The FeCl₃ solution is prepared by adding 0.2905 gm of FeCl₃ (or 0.4842 gm FeCl₃.6H₂O) to 1 liter distilled water and should also be refrigerated.

RC⁻ chlorosome isolation (Gerola, 1986) starts with cells concentrated (600 ml) by centrifugation at 3,600×g for 60 min. 2M NaSCN with 10 mM ascorbic acid in 10 mM Pi buffer (6.5 ml monobasic: 43.5 ml dibasic phosphate buffer per liter) was added to the weighed pellet in 4 ml/gm amounts. Cells were homogenized 10× in a cell disruptor/homogenizer (Fisher Scientific). Disruption of cells was performed by (one) pass in a 4° C. stored French Press (ThermoSpectronic) cell with 20,000 psi. DNAse I (Sigma) was added and the solution was incubated for 30 min at room temperature. The solution was passed through the cell two more times.

Cell debris was removed by pelleting at 3,600×g for 1 hr. A continuous sucrose gradient was established by placing 2.0 ml of a 40% sucrose in the NaSCN buffer in a tube and layering on 3.0 ml of a 10% sucrose solution. The tubes were placed, horizontally, into a dark, 5° C. storage until use (48 hrs later). The addition of 1.2 ml chlorosome solution to the top and ultracentifugation at 144,000×g for 18 hrs was started. Bands were collected by removal of the top band (by color), then removal by 1 ml at a time until the pellet was reached. The pellet was collected by addition of 1 ml to the tube and slight sonication to homogenize the pellet).

After isolation of the RC⁻ chlorosomes from C. aurantiacus whole cells, the chlorosomes were (at various dilutions into Tris buffer) tested as in the above methods. This was performed in order to assess quality control by comparing spectral data (on absorbance) and relative output (emission).

Surface effects (of the substrates) were tested by contact angle goniometry. This required a flat substrate (of sufficient surface area) to be placed on a Rame-Hart NRL Contact Angle Goniometer and test the contact angle with solution of known surface energies; Solution droplet formation was done with a syringe and about 100 μl droplet. Images were taken with the instruments CCD and using the RHI Imaging 2001 software to capture the digital pictures. Analysis can be done with the software. As well, as images were printed out and results were manually verified.

Another technique utilized the evaporation procedure as well as an aqueous method to allow incorporation of the chlorosomes onto a glass surface. Both techniques start with taking 0.5 μl of a known concentration of chlorosomes and placing it onto a borosilicate glass coverslip (Fisher Scientific). In the evaporation method, evaporation, under vacuum, is performed overnight and then the sample is sealed onto a fluorescent antibody microslide (Fisher Scientific). In the physical adsorption method, the slide is prepared in the aqueous phase and inverted during sealing, thus allowing for ensuring a hydrated sample as well as diffusion of the chlorosomes onto the surface of the hydrophobic glass. Samples were also studied under laser scanning confocal microscopy (instrument from LEICA) to investigate orientation and function (stability) was observed with absorbance spectroscopy of the sample afterwards.

The engineering photonic devices had to be characterized. Using a modified NIST approach to calibrate the detectors, a system to develop sensitivity curves (to wavelength) was established. Each device was calibrated under similar conditions and intensities were varied to demonstrate intensity changes (if present) in the device. Finally, devices that were to be enhanced had to be selected. Known nonlinearities in traditional devices, such as the silicon PV's (or solar cells) were selected as optimal devices for enhancement.

These devices were stimulated by white light filtered with interference filters to provide wavelength control from blue to NIR. Intensity was adjusted and matched using a radiometer from International Light (IL-1700). Changing the intensity from 0.01 to 70 lux caused a dramatic shift in the blue region by use of NDF's on an optical table (Edmund Optical Division).

The counting and size information gathering was accomplished by several high-resolution microscopy techniques. Transmission Electron Microscopy (TEM) was performed by taking isolated chlorosomes and evaporating a 0.5 μl drop onto a bacitracin treated formvar coated grid (300 mesh). Negative stains of urinal acetate were used to enhance the images. Images were taken at the Life Science EM Facility at 25,000× magnification. Images were saved in jpeg format, inserted into MATLAB and data (size and counts) were taken. Calculations were then scaled to predict how many chlorosomes were in a 1 ml sample for each of three dilutions. Absorbance spectroscopy of these dilutions was also performed to correlate absorbance spectra to count for the given population using a Beckman DU-65 photospectrometer (technique mentioned later).

A final method was used to gather most of the counting data, namely, Field Emission Scanning Electron Microscopy (FESEM) at the Center for Solid State Electronics Research Center at Arizona State University on a Hitachi 4700 FESEM. Again, a hemocytometer technique was employed as an initial method that could be correlated to the others. Another technique used computer aided image processing to allow the chlorosomes surface to be assigned a ‘1’ or ‘white’ pixel value and the background a ‘0’ or ‘black’. Accounting for surface area (number of pixels) per chlorosome, histograms were made and counts were calculated via computer. The final technique was a modified ASTM method in which the surface is transversed from left to right, and top to bottom, counting chlorosomes until 100 is reached. Then the number of pictures required to reach ˜100 chlorosomes, the surface area of each picture, etc are accounted for and a final # chlorosomes/ml is calculated. Here, five concentrations (plus a distilled water control) were imaged using all three techniques and counts were correlated to ABS spectra as well to aid in future calculations or determinations. The stubs were prepared by evaporating 100 μl of the dilution onto a hydrophobic borosilicate glass disk, attaching the disk to a stub via tape and carbon coating the samples for a period of 10 minutes. The chlorosomes were diluted with Tris buffer at pH 8.0 and 10 mM NaCl, by addition of 0.788 gm Trizma HCl into 500 ml of DI water, under constant stirring. Meanwhile, add 0.605 gm of Trizma Base was added into 500 ml of DI water under constant stirring. Both solutions were mixed together and 0.9 gm NaCl was added while mixture was stirred thus making 1 liter of 10 mM Trizma buffer, pH 8.0 with 20 mM NaCl.

Another imaging technique, namely Atomic Force Microscopy (AFM) was performed by evaporating a 100 μl sample of chlorosomes (overnight in desiccant jar) onto a standard borosilicate coverglass. A Digital Instruments' Nanoscope III Multimode AFM was used in Tapping Mode (TMAFM) to image the chlorosomes at various dilutions. Again, the dilutions' absorbance spectra were taken prior to imaging. Prior to running the AFM experiments, a known liquid volume (400 μl) was taken from solution containing RC⁻ chlorosomes in DI water previously characterized via absorbance spectra (ABS=0.01@740 nm) and was evaporated onto a clean, optically clear glass disk with known surface area (113.1 mm²). The disks were made hydrophobic to enhance RC⁻ attachment and orientation due to theoretical studies performed by using a molecular modeling algorithm (Chou, 1977) that suggested that the baseplate region attached to the reaction center may be hydrophobic in nature. Tapping mode AFM experiments were conducted utilizing a small scan head (D head) to scan 1 μm² surface areas on both the control disks (no RC⁻ chlorosomes deposited) and test disks (RC⁻ chlorosomes deposited).

Ranges of dilutions were made by serial dilution of the stock chlorosome sample. Each dilution was placed into a standard cuvette (using a blank of Tris buffer) and full (400-900 nm) absorbance readings were gathered (via an RS232C port) onto computer and analyzed and plotted in MATLAB. At this point, selection of a non-pigment wavelength (650 nm in the case of the chlorosomes) was made to use in correlating absorbance to the previous counts made on each dilution and then plotted. This wavelength was selected for its non-photosynthetic (non-optically active) properties and consistent nature between different growth conditions during the counting experiments. Hence, a calibration curve was made between counting and absorbance for a series of dilutions of chlorosomes.

The TEM images were placed into the Image Processing Toolbox for MATLAB for sizing measurements and calculations processing. The scalebar was measured (in the number of pixels across it to length) and then correlated to the size of the bar so a conversion could be made for length and width of chlorosomes. The command ‘ginput’ was used to grab the distal ends of the chlorosomes and utilizing the Pythagorean Theorem: a²+b²=c², measurements of length and width were made by selecting random chlorosomes and measuring 5 per image. 25 chlorosomes from each image were selected and measured to ensure statistical distributions could be made. Counts (per μm²) at this point were also made and calculations were made to correlate to a count per ml of each dilution and then related to the corresponding absorbance spectra.

In the AFM and FESEM studied, the images were taken and saved in jpeg format for processing in MATLAB as was done in the TEM images. Size was verified but in these techniques, counting was the main objective. The same process of taking the counts in an area and re-calculating what the count per ml was performed on many dilutions to enable a more accurate count (and correlation to absorbance data).

The same samples were sent to Protein Solutions Inc. DLS was run on the samples. Later (after purchase of a DLS system) 20.0 μl of each sample was injected via a gas-tight syringe into the quartz cuvette and readings were taken at 2 Acq/sec. Data filtering was performed to minimize dust events but capture the quickly diffusing small particles. The Dynamics V6 software developed the autocorrelation curves and produced the polydisperse plots of R_(h) versus % mass for each sample.

During the AFM studies, the hydrophobicity of the baseplate was studied by utilizing differently treated coverglass in LSC. Untreated coverglass remained very hydrophobic, with a critical surface tension around 12 dynes/cm, a surface tension of 32 dynes/cm, and a contact angle (for DI water) of 41°. A heat treatment (450° C. for 4 hours) allowed for the coverglass to pass through the glass-transition (T_(g)) temperature and delivers the surface into a hydrophilic state (surface tension close to 12 dynes/cm and a contact angle of ≦1° for DI water). The samples were placed onto the surfaces (in a laminar flow hood to reduce contamination) and evaporated under vacuum over night. Imaging was performed within 36 hours of evaporation so that the chlorosomes would not swell (degrade). Images were taken and stored as jpeg format files and processed in MATLAB as with the other imaging techniques. Unusual formations or interactions at the surface were also imaged in the pictures. Placement of the concentrations required to make certain percent coverages into the microwells were done with an incubation time necessary for physical adsorption. The time was a predicted time based upon diffusion coefficient of the chlorosomes (as measured by DLS) and the path length. The final, assembled coverglass and microwells were sealed with a two-part epoxy and allowed to cure overnight.

The first stability test for the isolated chlorosomes tested storage under two conditions. A ‘fresh’ sample was maintained for use in 7° C. freezer and a long-term (or later called ‘frozen’) sample was placed in liquid nitrogen (LN₂). Initial degradation was noted in the samples and can be clearly seen (at the monomeric 670 nm absorbance peak) in the absorbance spectra of the ‘fresh’ sample. Emission spectra were even gathered to see if a decrease in emission occurred.

In intensity related photodegradation, concentrations were matched between all samples (six different intensities were investigated) by absorbance readings. Therefore, a series of experiments were designed and run with chlorosomes, with and without reaction centers, in solution, to see this effect. Samples were diluted to 1:100 of the original stock into Tris buffer. 2 ml each were separated out for 6 different light conditions. Light intensity was varied by the use of filters, no filter, or no light such that % Transmissions were 0% T, 14% T, 36% T, 53% T, 68% T, and 100% T and measured (photometrically). The light source was a standard 100-watt white light bulb. Degradation was recorded at times when 5, 10, and 15% degradation was noted. Degradation was quantified by noting a percent decrease in the 740 nm absorbance. The samples were continuously illuminated and at specific time intervals, absorbance readings were taken. Degradation of the 740 Bchl c Q_(y) band was measured by (1) peak height from start to finish and by (2) integration of the area under the Q_(y) band. Times were marked when 5, 10, and 15% degradation of the peak were attained. A control sample (buffer) was also held under the same illumination and used as the blank in the photospectrometer.

Next, in intensity related to concentration photodegradation experiments, various concentrations of chlorosomes (in 2 ml) were degraded by a similar white light (at fixed intensity). From these sets of experiments minimum photostress terms were calculated to determine a 0% degradation intensity (and time). Again, the 740 nm peak height provided a measure (by absorbance spectra) of photodegradation over time.

Another mode of destruction of the photo-stability of the chlorosomes could be simple denaturation (by acidicity) by the buffer. Therefore, buffers (with a varying pH) were made from pH 2.0 to 12.0 and 1 ml of each was added to 1 ml of a chlorosome stock solution. Absorbance spectra as well as R_(h) were measured for each sample. The R_(h) was measured by testing 20 μl of the sample in the DLS system.

Heat (or temperature) induced photodegradation or denaturation was also explored and tested. Starting at room temperature, a water bath holding a vial of chlorosomes was brought to near boiling over a period of hours. During the experiment, absorbance readings were taken at about every 5-10° C. and degradation was calculated as mentioned previously.

Another mode of destruction of the photo-stability was tested by increasing the concentration of the chlorosomes, in solution to see if concentration aggregation could be attained. This was accomplished by use of concentration filters and measured by R_(h). 15 ml of sample was concentrated down to differing volumes and the filtrate (buffer) was removed leaving a more concentrated sample. Then 20 μl of sample was removed for DLS measurements after absorbance spectra were taken to ensure viable sample and perform chlorosome counting.

A final experiment to show another mode of destruction of photo-stability was the addition of a competitor (for absorbance of blue light). Carotenoid solutions from the isolation procedure were reintroduced into the chlorosome sample (by dilution) and emission measurements were taken. Side control experiments were performed by addition of buffer alone. Stimulation was made by the RF-1501 Shimadzu spectrofluorometer and emission was measured on a photodiode after passage through an 800 nm interference filter (so that scatter and excitation energies could be removed). This also allowed for ratios of the Bchl c to a, Soret, and carotenoid peak to be calculated and compared for potential enhancement calculations for the hybrid well experiments.

The initial step in manufacture of the hybrid wells is the chlorosomes (or controls) themselves. First the isolated solution of chlorosomes had to be measured in order to determine the actual number of chlorosomes (per ml) in the solution. For the controls, this was done by number of molecules based upon molecular weight (fluorescein) or counts supplied by manufacturers (unlabeled and labeled particles). Next, calculations (and dilutions) had to be made in order to develop a varying percent coverage. When all coverages (and dilutions) were made, the procedure was the same. Place 20 μl of sample onto a hydrophobic coverglass and incubate in the laminar flow hood, in the dark, for at least 10 minutes. This gives the chlorosomes (or controls) enough time to physically adsorb onto the borosilicate glass surface. Next, invert the coverglass and place on top of the microslide holder centered on the frosted ring (1 cm in diameter). Sealing is performed by use of a 2-part (optical grade) epoxy. The samples are then placed in a microslide holder and stored over night (at least 24 hours) in the dark at room temperature. Further storage should be done at 5° C. in the dark.

The biophotonic hybrid device had to then be assembled, using the various interfacing techniques to integrate the chlorosomes (and controls), in a controlled, patterned array with the silicon (Si) photovoltaic (PV) photocell. Once fabricated, the device parameters or specifications had to be tested. These include: maximum output, time-response (or rise time), spectral sensitivity, intensity sensitivity, temperature sensitivity, and device lifetime.

The device was fabricated by utilizing physical adsorption immobilization to interface chlorosomes (on a glass substrate/microslide with well) to the Si PV photocell. The components were interfaced (mechanically) by a self-built optical chamber made from acrylic sheet. The microslide port was milled into one piece, holes were drilled for the fiber optic bundle and the Si PV detector. Accessory ports/chambers were made to fit 25 mm filters such as additive (or subtractive) and NDF for wavelength and intensity control, respectively. The whole apparatus was black felted to reduce external light leakage. Power was supplied using a standard variable power supply (for the LED) and the Si PV was monitored utilizing a digital multimeter (DMM).

In this arrangement, device parameters such as maximum output, time-response (or rise time), spectral sensitivity, intensity sensitivity, temperature sensitivity, and device lifetime were tested. Maximum output was monitored by allowing the device sufficient time to go from 0 millivolts (mV) to maximum for that particular LED intensity. The difference was recorded and compared to when no sample is introduced. This ratio was defined as normalized relative output in the following sections. Response time is defined as the time that required going from 0 to 90% of the final value during a switching on stage.

This was performed by timing a device versus the standard Si PV detector (no device attached). Spectral sensitivity was performed by replacing the LED with various colored LED's that covered the visible spectrum as well as into the near infrared (NIR). Voltages were recorded, and normalized relative outputs were made on the final device. All devices were tested using 470 nm, 735 nm, 880 nm, and white LED's (full spectra). Intensity sensitivity was verified using the blue (470 nm) LED since this was the wavelength of choice for enhancement.

Stability was verified by absorbance spectroscopy (400-900 nm) and degradation was recorded. Temperature of operation was also investigated and degradation was also monitored so that an operational range of temperatures could be established. In these experiments, data was also obtained to establish device lifetimes under such ‘operational’ conditions. The lifetime was determined by seeing what conditions led to device degradation and the time required to reach that point.

The devices (ranging from low to high percent coverages) were tested under LED illumination and ratios were made to the same detector under the same illumination (minus the hybrid wells) and percentages have been calculated. This percent enhancement signifies when a hybrid well device increases the measured output (and by how much percent) over the stand-alone configuration. The rise time (the time it takes to get from 10% to 90% final voltage) was measured and compared between the hybrid well devices to the stand-alone detector. This was accomplished with a stopwatch and DMM. White light LED (visible light) stimulation of a series of percent coverages have been conducted and compared to monochromatic results. White LED's were implemented into the device apparatus and run at a few intensities and on Si PV as well as Si TP. Device enhancement was not further enhanced by the addition of the full spectra light, in fact, red band quenching might have been recorded as noted by others (Klar, 2000) in other systems and setups. Using monochromatic light as the stimulation source intensities were run at different levels sufficient for device detection but lower than saturation (of device or hybrid component). Again, full range of percent coverages were evaluated and replicates run. Again, the detector's response (without the hybrid layer) was used as a point of reference in determining percent enhancement.

Other tests included data gathered from solution effect studies only. Temperature effects were determined by the previous experiment in which the chlorosomes' photostability to temperature changes was determined. Being in a bulk system in a water bath controlled environment fitted the design parameters of the hybrid well experiments and throughout testing, no experiments were conducted outside the 25-100° C. range. A series of hybrid devices were constructed and tested (positively) over a large period of time (and intensities). Further experimentation was conducted on these samples throughout the research endeavor until no (positive) responses were seen. Afterwards, the absorbance spectra of a few high percent coverages were measured using a home-built slide holder in the DU-65 Beckman Photospectrometer in order to check the status (photostability) of the chlorosomes.

Forced Adaptation

Inconsistent results initially plagued experiments leading to the development of the hybrid device of the invention. As noted, C. aurantiacus is self-adapting. This meant that chlorosomes taken from the same growth of cells could not be relied upon to behave consistently.

To overcome this lack of consistency force-adapted C. aurantiacus was developed having the performance desired. To this end, and because of the number of environmental variables involved in the growth of the cells and their substituent chlorosomes, a design of experiment (DoE) technique was employed to arrive at chlorosomes that performed well and consistently.

In relation to biophotonic device design, there are many pertinent issues in each stage of the design. Variables encountered throughout the entire process of making the hybrid photovoltaic device that are capable of affecting results are set out in Table 2. The design of the product stage (as tested by validation techniques) requires that the device be tested via an appropriate light source type and wavelength (such as a 470 nm LED) or a incandescent light bulb with correct interference filter (470 nm), and can be projected to the surface of the device with or without the aid of light pipes such as waveguides and/or fiber optics. The intensity that the device is stimulated with must also be of appropriate intensity as controlled by the voltage applied to the source (LED for example), a neutral density filter (NDF), or other means. Stimulation time must also be accounted for since the time of stimulation and intensity will correlate to a certain photostress that the device can handle before irreversible damage is done to the biohybrid layer. A controlled environment (for validation purposes) is also necessary (a dark room or constant intensity area), as well as selection of an appropriate measuring device, such as a high impedance digital multimeter (DMM) for photovoltaic devices for example.

Before the device can be tested however, materials must be acquired/produced and synthesized. These stages involve: growth of the bacteria and alteration (if any) of the chlorosomes. In this example, synthesis is governed by production in that changes in the chlorosomes can be induced by the growth period factors. Some of these factors include: intensity of light source, light type and wavelength (incandescent, LED, fluorescent); media (pH, temperature, components or strength); number of days allowed for growth (before isolation or media exchange); bottle-fill volume; and temperature. Some of these factors directly influence important design characteristics such as Figure of Merit (FoM), chlorosome size, photostability, and indirectly quenchers, to list a few.

Processing of the chlorosome requires isolation of the chlorosomes from the whole cell walls. This is done using a procedure well documented in the literature although certain factors do arise in the process. There are different procedures used to isolate chlorosomes without the reaction centers (RC⁻) versus those with (RC⁺). The solvents, agents, and buffer types used in the procedure are also very important and factors such as (the type, molarity, ionic strength, pH, and strength) all come into play. These factors will affect the state of aggregation and purity (and successful use) of the isolated chlorosomes.

Manufacture of the chlorosome layer is the step whereby means of immobilization (namely physical adsorption) a monolayer (or percent thereof) is deposited onto the surface of a substrate (borosilicate glass). Important factors for successful devices include: the fabrication conditions (temperature, incubation time, Light ON/OFF, and in the laminar flow hood); sealing method; concentration, volume, and % coverage (and hence interparticle distances); droplet placement (on the coverslip or in the well); and coverslip hydrophobicity, which all relate to chlorosome orientation (facing Si PV or LED).

Again, product final assembly is addressed above. However, other issues pertaining to lifetime of device and/or other issues such as post fabrication storage include factors of temperature, light intensity (and quality—i.e. wavelength) and # days, to name but a few.

TABLE 2 WELL ISSUES Growth Isolation Sample Fab Sample Run Intensity Procedure Fab conditions Light Source type Temperature LED Incubation time Light bulb Light ON/OFF W/wout F.O. laminar hood Media Buffer Buffer Light Intensity Type Type V_(applied) Molarity Molarity NDF used Ionic Strength Ionic Strength Measure tech. pH pH Stim. Time Temperature Temperature pH Aggregated? Sealing method Light wavelength V_(applied) LED Light type Type (RC ±) Post fab storage Holder Incandescent Temperature Sample holder LED Dark LED + NDF Fluorescent # days SiPV + LPF Days of growth Purity % coverage Intensity control Bottle Volume Volume Detector Temperature Droplet placed DMM used On coverslip 9 V (new) In Well High Imped. Wavelength Coverslip Orientation hydrophobicity Facing SiPV Facing LED Concentration Red LPF used Voltage Applied NDF used Room lights ON/OFF Stimulation time

In one stage of the development of the preferred exemplary procedures described in the above example, a multiple input, multiple output environmental chamber 150 (MIMO/EC) was constructed as diagrammed in FIG. 22.

Nine compartments 155 through 163 were provided. Light intensity increased from left to right across the three columns of compartments and temperature increased from bottom to top across the three rows of compartments. Within the compartments three differing volumes of media were contained. Consequently, 27 combinations of variables were able to be tested. FIG. 23 illustrates an environmental chamber of this kind. On its door 170 multiple shelves 172 are supported and have openings to retain culture-containing rest tubes or containers. Vertical dividers 174 separate the compartments 155-163. Horizontal dividers 176 separate the compartments vertically. Light bulbs, one of which is shown at 177 provide illumination. A series of fans 179 regulate temperature.

Ordinarily in biology research is conducted on the OFAT, one factor at a time method. Here, the DOE approach permitted the three factors, light intensity, temperature and media to air volume ratio to be tested at three levels with three replicates and an additional three centerpoints and a total of 27 experiments (23×3+3=27). The DOE technique allows for correlation of data statistically easier than OFAT or best-guess approaches. It reduces the total number of experiments, allows for a good, thorough experimental design. It allows for error to be quantified and it can distinguish if factors have any to no effect or if interaction among factors occurs. Here, the response, the output under study, was concentration (by absorbent spectroscopy) after three days' growth.

EXAMPLE

A factorial design was chosen to quantify the relative importance of interaction between light intensity, temperature, and volume of media. The approach used was Design of Experiments (DOE). This method allows for data to be gathered in a way to avoid error by establishing an experiment protocol and quantify error in a mathematical way. The regression method that was used was the analysis of variance (ANOVA) technique. This tool (DOE) allows for data to be gathered at normal conditions (centerpoint) and at extremes (above and below the centerpoint). Analysis is based on quantifying effect and probability of effect of a factor or interaction on the output variable.

Cell Culture Stock

The cell culture stock was prepared for testing as in [1] and the MIMO/EC DOE culture incubation apparatus was also used. The data was gathered from nine strands that all came from a centerpoint grown stock (cultured at the centerpoint for 14 days). The data was gathered (randomly) at the end of a three-day growth cycle period and placed into the Stat-Ease™ software for analysis. Three replicates at each corner were taken as well as five centerpoint readings.

Pigment Protein Content Determination

The pigment protein content was deduced by taking absorbance spectra from 650 nm to 900 nm on each sample. This was done with a Beckman DU-65 photospectrometer. Then a ratio (R₁) was calculated by dividing the absorbance at 740 nm by that at 808 nm. Then another ratio (R₂) was calculated with the 740 over 866 nm peak absorbance readings. In this experiment, pigment protein content was desired to see an increase (larger chlorosomes).

Statistical Analysis Approach

The DOE approach used involves seven steps in order to perform the experiment. The first step involves defining the problem statement. Here it was desired to investigate which factors could increase the pigment protein content of the chlorosomes. Next, the choice of the factors, which may influence pigment protein content, had to be chosen. Also, the levels of these factors had to be established. The factors that were chosen, and their levels can be found in Table 3. below.

TABLE 3 Low, Centerpoint, and High Factor Levels for DOE Experiment Factor Low (−) Center High (+) A = Temp 36° C. 48° C. 60° C. B = Intensity 50 lumen 270 lumen 490 lumen C = Media 5 ml 7.5 ml 10 ml

The next step is to identify the output variable(s) to be studied. Since the change in pigment protein content was desired to be analyzed, the ratios of the 740 to 808 and 740 to 866 nm peak absorbances were chosen. The ratios were designated with a R₁ for the 740/808 and a R₂ for the 740/866 ratio. Since the choice of factors and levels were as stated, a 23 factorial approach was chosen. In this approach, three replicates and five centerpoints were chosen also. The experiment was run at the end of a three day growth period and data was gathered in a random fashion. Since replicates were used the data analysis will not include determination normal % distribution plot and the analysis will really be based on the ANOVA tables. Interaction between factors was determined from the ANOVA as well as the interaction graphs provided by the software. Finally conclusions must be made based on the analysis and results.

The R₁ ratio developed strong effects due to each individual factor and the interaction between Temperature and % Volume. All other interactions were insignificant when compared to these four factors/interaction. This can be seen in the ANOVA table in Table 4. The normal % probability plot and interaction plot (between Temp and % Vol) can be found in FIGS. 11, 13 a and 13 b. Based on the analysis, the highest level for the R₁ ratio would be with bacteria grown under the following conditions: low temperature, low light intensity, and high % volume.

TABLE 4 ANOVA Table for experiment. Note DF represents degrees of freedom and CE is coefficient estimate. An appropriate prob>|t| was chosen to be 0.01 for this output variable therefore A, B, C, and AC have an effect on this output. Factor CE DF Error Prob>|t| Intercept 1.23 1 9.913 × 10⁻³ A-Temperature −.032 1 9.913 × 10⁻³ .0041 B-Light Intensity −.037 1 9.913 × 10⁻³ .0013 C-% Volume −.044 1 9.913 × 10⁻³ .0003 AB .027 1 9.913 × 10⁻³ .0118 AC −.056 1 9.913 × 10⁻³ <.0001 BC .015 1 9.913 × 10⁻³ .1372 ABC −.021 1 9.913 × 10⁻³ .0502 Centerpoint .41 1 .024 <.0001

The R₂ ratio developed strong effects due to only temperature and no interactions. All other factors and interactions were insignificant when compared to temperature (see Table 5). The normal % probability plot and interaction plot (between Temp and % Vol) can be found in FIGS. 13 a and 13 b. As shown in FIG. 14 a, the results are so close to the linear line that they are deemed insignificant except for temperature. Even the interaction plots (FIG. 14 b) showed slight interactions. Note how the lines cross but the error bars overlap so that these lines could in fact be parallel and therefore non-interacting. The highest level possible for the R₂ ratio would be with bacteria grown under low temperature.

TABLE 5 ANOVA Table for experiment of R₂ ratio. Note DF represents degrees of freedom. An appropriate prob>|t| was chosen to be 0.1 for this output variable therefore A, B, C, and AC have an effect on this output. Coefficient Factor Estimate DF Error Prob>|t| Intercept 1.23 1 .047 A-Temperature −.11 1 .047 .0321 B-Light Intensity .032 1 .047 .5080 C-% Volume .006 1 .047 .8990 AB .049 1 .047 .3050 AC −.035 1 .047 .4651 BC −.036 1 .047 .4516 ABC −.077 1 .047 .1181 Centerpoint .51 1 .11 .0002

It is interesting to note from the results that the response variables (namely R₁ and R₂) are not dependent upon the same factors. R₁ is sensitive to temperature, light intensity, and % volume and the interaction of temperature and % volume. However, the R₂ ratio is dependent upon only the temperature during growth. This ratio was long believed to be only dependent upon light intensity but temperature was more significant. This may be due to the fact that the real dependent output is the R₁ ratio. If the bacteria are grown under those conditions and R₁ changes, R₂ must change as well but not vice-versa.

It is also possible that the temperature affects only the 866 nm molecules and light never changes growth (within limits selected in this study). Another, stronger argument is that since the natural funnel-like energy transfer in the chlorosome (from 740 to 795 to 808 to 866 nm molecules) protects the molecules further down the chain (like the 866 Bchl a) from being sensitive to factors such as light. At the same time, these molecules are still protein based and very dependent upon temperature effects.

TABLE 6 DOE experiment calculated data for pigment-protein growth/development ratios over a period of 6 transfers (3 weeks approximately). condition ratio Nov. 18, 1997 Nov. 21, 1997 Nov. 25, 1997 Nov. 28, 1997 Dec. 2, 1997 Dec. 9, 1997 −−− 740/808 1.3182 1.2 1.1111 1.2174 1.25 1.0526 740/866 1.45 1.3333 1.1111 1.4 2 1.3333 −−+ 740/808 1.2381 1.1071 1.16 1.28 1.2308 1.1667 740/866 1.3929 1.1273 1.2889 1.4545 1.4545 1.25 −+− 740/808 1.1579 1.0345 .9231 1.1842 1.0526 1.017 740/866 1.2571 1.0526 .8571 1.2857 1.0909 1.3 −++ 740/808 1.2353 1.375 1.2027 1.1667 1.2 1.4 740/866 1.377 1.5068 1.3692 1.3462 1.3548 1.4848 +−− 740/808 1.3333 1.2222 2.0732 2.1458 1.88 1.125 740/866 1.4545 1.375 2.2667 2.4235 2.0435 1.1538 +−+ 740/808 1.6 1 1 2.3368 1.7412 1.9759 740/866 2 1 1 2.6118 1.9221 2.2162 ++− 740/808 1.2857 1.0952 1.1579 1.2917 1.2687 1.25 740/866 1.4062 1.2778 1.2941 1.4531 1.4167 1.3514 +++ 740/808 1.1111 1 1 1.3049 1.2273 1.3929 740/866 1.3333 1 1 1.4079 1.35 1.56 000 740/808 1.7105 1.7901 1.7901 1.5854 1.686 1.5224 740/866 1.8571 1.9079 1.9595 1.6667 1.7262 1.619

Changes could clearly be seen from one transfer to the next. This suggests a forced evolution situation. The bacteria are being forced to survive in a hostile environment.

Take the +−− bacteria. In the first two transfers, it looked like it was dying and then by the third environment, some cells have adapted to the different environment and grown. Remember that the +−− was high temperature, low light intensity, and low amount of food source. Other changes can be seen in this sequence of pictures but clearly, this was the most significant.

The light intensity and the light-temperature interaction factors had coefficients of only one half the temperature factor in the 740 nm variable. This contrast was particularly apparent in those response variables that do not have photosynthetic activity. There is clearly a correlation between the light factor, the light and temperature interaction, and the absorbance of Bchl c (740 nm). Since the other response variables are mostly dependent on temperature, their changes can be primarily attributed to the change in absorbance which results from increased and/or decreased concentration of cells. Because cellular membrane components have an absorbance of 650-700 nm, the concentration of cells in each sample can be determined from the absorbance data in this region. By normalizing the data, it is possible to extrapolate the Bchl c absorbance for individual cells. This is the next logical step in analyzing the data.

A method was developed to establish a faster process to count whole cells. A modified hemocytometry counting technique was used to count whole cell C. aurantiacus concentrations (per unit length of 10 μm), and absorbance data was gathered as three replicates of: 1:1, 1:1.1, 1:1.5, 1:2, 1:3, 1:4, 1:5, 1:0, and 1:50 dilutions were made. Full spectra (absorbance) data was gathered for each dilution, as in FIG. 15 a. Each replicate was run to minimize instrument and operator error, FIG. 15 b and peak data was gathered and averaged at 650, 740, 808, and 866 nm. The samples were then counted on an optical microscope using a standard red blood cell counting technique and a hemocytometer. In this fashion, curves were developed for absorbance at 650 and 740 nm and cellular counts with error bars as in FIGS. 16 a and 16 b.

Counting Chlorosomes

For the purposes of characterization and conformity in preparing the hybrid devices contemplated, determining the quantity of chlorosomes coating the cover glass hydrophobic surface was important. Absorbance of light was correlated to the density of chlorosomes as illustrated in FIG. 17. The calibration plot of FIG. 17 plots chlorosome count against chlorosome absorbance at the 650 nm wavelength. The 650 nm wavelength is chosen rather than a wavelength where absorbance of the chlorosome exhibits a peak because the absorbance at those wavelengths exhibiting a peak in the absorbance spectrum vary from one chlorosome to another depending, inter alia, on environmental factors effecting the growth of the bacterium from which the chlorosome was taken. The 650 nm wavelength absorbance, then, is linearly related to chlorosome count and not another variable.

In the exemplary preferred embodiment employing the chlorosomes of C. aurantiacus to enhance SiPV performance, chlorosome percent coverage of the SiPV's light receiving surface (or the overlying borosilicate glass) is important as demonstrated by the FIG. 19 plot of percent enhancement against percent coverage. Ideally, in this particular embodiment at least, coverage should be in the 4 to 7% range and preferably about 4%.

To arrive at percent coverage, accurate counting of the chlorosomes becomes important.

FIG. 1 is a conceptual block diagram that indicates the design and development of a hybrid device of the nature of the enhanced photovoltaic cell described above. At each stage of development multiple variables entered the design process. This is tabulated, as well, in Table 2. From this it will be seen that a robust program such as the design of experiments program that permits the assessment of multiple variables and their interaction is an enabling design tool in arriving at a final product that meets the objectives of high performance, robustness, scalability, energy interactivity and adaptability.

In the case of the enhanced hybrid photovoltaic device many pertinent issues arise at each stage of design. At the product stage, the device needs to be tested using an appropriate light source and wavelength, such as a 470 nm LED or an incandescent light bulb with a correct interference filter yielding 470 nm wavelength. Intensity is a variable. The use of suitable light waveguides or fiber optics may be a variable to consider. Stimulation time must be taken into account since it and intensity will correlate to a certain photostress that the device will be able to handle or not handle if irreversible damage is to be avoided. Controlled environments and appropriate measurement devices are to be chosen.

Before the device can be tested, however, materials must be acquired, and/or produced as by systhesization. Here the stages involve growth of the bacteria and alteration if that is needed to alter the chlorosomes that are to be employed. As has been seen, factors involved in the growth period can affect the chlorosomes, either beneficially or not. Some of these factors include light intensity, light type (i.e. incandescent, LED or fluorescent) and wavelength. Media (its PH, temperature, components, and strength) can affect chlorosome yielding bacteria development. The number of days allowed for bacterial growth (either before isolation of the bacteria or before an exchange of media) is another factor. Bottle-filled volume or, as has been shown, percent media to air and temperature are factors, as well. Some of these factors directly influence important design characteristics such as “Figure of Merit,” discussed below, chlorosome size, chlorosome photostability, indirect quenching, etc.

Processing of the chlorosome requires isolation of the chlorosomes from the whole cells. As indicated above, this is done using procedures well documented. Nevertheless certain factors need to be taken into account during this process. These are the different procedures used to isolate chlorosomes without the reaction centers (i.e. the RC⁻ chlorosomes vs. the RC⁺ chlorosomes). Solvents, agents and buffer types used in the procedure are also important, and factors such as the type, molarity, ionic strength, pH and strength of these all come into play. These factors will affect the state of aggregation impurity of the isolated chlorosomes, and consequently the ultimate success of the design.

Manufacture of the chlorosomes layer is the step whereby means of immobilization (which is to say physical absorption) of a monolayer (or a percent of a monolayer) is deposited onto the surface of the substrate such as the borosilicate glass. Here, important factors for successful devices include the fabrication conditions of temperature, incubation time, lighting (on or off) and operation of a laminar flow hood. Sealing method, concentration volume and percentage of coverage enhance interpartical distances, dropment placement on the cover slip or in the well, and cover slip hydrophobisity all bear on chlorosome placement and orientation (i.e. either facing the SiPV or the LED in the preceding exemplary arrangements).

Final product assembly involves many of these same factors just raised, others described above and other factors commonly encountered in product production. Further concerns relate to device lifetime, postfabrication storage including temperature light intensity, type and wavelength are additional concerns.

From the above, then, it should be evident that best guess or one factor at a time approaches to design and development pale in comparison to the DOE approach.

Figure of Merit

“Figure of Merit” (FoM) is a concept employed widely and in many disciplines, although ordinarily not where biological matters arise. In the present invention a biophotonic Figure of Merit was devised to quantify chlorosome performance.

Bearing in mind the chlorosome functioning as conceptually diagrammed in the block diagram of FIG. 21 a, the following Figure of Merit was devised.

${FoM} = {\frac{\% \mspace{14mu} T_{440{({{Bchl}\mspace{14mu} c\mspace{14mu} {Soret}})}}}{{\% \mspace{14mu} T_{440{({{Bchl}\mspace{14mu} c\mspace{14mu} {Soret}})}}} + {\% \mspace{14mu} T_{460{({Carotenoid})}}}}*{\frac{\% \mspace{14mu} T_{795{({{Bchl}\mspace{14mu} a\mspace{14mu} {Baseplate}})}}}{\% \mspace{14mu} T_{740{({{Bchl}\mspace{14mu} c\mspace{14mu} {Oligomeric}\mspace{14mu} {Qy}})}}}.}}$

This FoM takes into account the total transmittance of the Bchl c Soret at 440 nm as compared to the total Soret and corrotenoid 460 nm transmittance and the baseplate Bchl a transmittance at 795 nm as compared to the Bchl c oligomeric transmission at 740 nm.

Engineering to a Figure of Merit in the exemplary embodiment of this invention was calculated to yield 160% of the Vout response of the original silicon photovoltaic cell at a Figure of Merit of 1.0. The actual improved output was measured at 157%.

Although preferred embodiments of the invention have been described in detail, it will be readily appreciated by those skilled in the art that further modifications, alterations and additions to the invention embodiments disclosed may be made without departure from the spirit and scope of the invention as set forth in the appended claims.

Isolation, Separation and Harvesting Techniques for Light Antennas and Subunits

Books:

-   Chlorophylls, Hogo Scheer, CRC, 1991. -   Light-Harvesting Antennas in Photosynthesis, B R Green and W W     Parson, eds. Kluwer Academic Publisheing (Dordrecht: Kluwer), 1991. -   Photosynthesis: Photobiochemistry and Photobiophysics, Bacon Ke,     Springer, 2001 -   Oxygenic Photosynthesis The Light Reactions, Donald R. Ort,     Charles F. Yocum, Iris F. Heichel, Springer, 1996. -   Chemicals from Microalgae, Zvi Cohen, CRC, 1999 -   Physicochemical & Environmental Plant Physiology, Park S, Nobel,     Academic Press, 2005 -   Molecular Biology of Membranes: Structure and Function, Howard R.     Petty, 1993 Structure of Phototrophic Prokaryotes, John F. Stolz,     CRC 1990

Journals:

-   Yamnaka, G., et al., Molecular Architecture of a Light-harvesting     Antenna Isolation and Characterization of Phycobilisome Subassembly     Particles, J Biol. Chem., 257(8): 4077-4086, 1982. -   Lundell, D and A N Glazer, Molecular Architecture of a     Light-harvesting Antenna Structure of the 18 s Core-rod Subassembly     of the Synechococcus 6301 Phycobilisome, J Biol Chem., 258: 994-901,     1983. -   Montaño GA, Wu H-M, Lin S, Brune D C and Blankenship R E (2003)     Isolation and characterization of the B798 baseplate     light-harvesting complex from the chlorosomes of Chloroflexus     aurantiacus. Biochemistry 42:10246-10251. -   Simidjiev, I., et al., Isolation of Lamellar Aggregates of the     Light-Harvesting Chlorophyll a/b Protein Complex of Photosystem II     with Long-Range Chiral Order and Structural Flexibility -   Montaño, G A, Bowen B P, LaBelle J T, Woodbury N R, Pizziconi V B     and Blankenship R E (2003) Characterization of Chlorobium tepidum     chlorosomes. A calculation of bacteriochlorophyll c per chlorosome     and oligomer modeling. Biophys. J. 85: 2560-2565. -   Blankenship R E and Matsuura K (2003). Antenna complexes from green     photosynthetic bacteria. In: Light-Harvesting Antennas, B R Green     and W W Parson, eds. Kluwer Academic Publishing (Dordrecht: Kluwer),     195-217. 1991. -   Hu, D. and Blankenship, R. E. (2002) Rapid one step purification of     the BChl-a containing FMO-protein from the green sulfur bacterium     Chlorobium tepidum using a high efficiency immunomatrix, Photosynth.     Res., 71, 149-154 -   Frigaard, N-U, et al., Isolation and characterization of     carotenosomes from a bacteriochlorophyll c-less mutant of Chlorobium     tepidum, Photosynthesis Research, 86:101-111, 2005. -   Qian, P., et al., Isolation and Purification of the Reaction Center     (RC) and the Core (RC-LH1) Complex from Rhodobium marinum: the LH1     Ring of the Detergent-Solubilized Core Complex Contains 32     Bacteriochlorophylls, Plant Cell Physiology, 41(12): 1347-1353,     2000. -   Cogdell, R. G., 1. Durant, J. Valentine, J. G. Lindsay, and K.     Schmidt. 1983. The isolation and partial characterisation of the     light-harvesting pigment-protein complement of Rps. acidophila.     Biochim. Biophys. Acta. 722:427-455. -   Cogdell, R. J., and A. M. Hawthornthwaite. 1993. Preparation,     purification and crystallization of purple bacterial antenna     complexes. In The Photosynthetic Reaction Center, Vol. 1. J. R.     Norris and J. Deisenhofer, editors. Academic Press, New York. 23-42. -   Clayton, R. K., and B. J. Clayton. 1981. B850 pigment-protein     complex of Rhodopseudomonas sphaeroides: extinction coefficients,     circular dichroism, and the reversible binding of     bacteriochlorophyll. Proc. Natl. Acad. Sci. U.S.A. 78:5583-5587. -   Cogdell, R. J., and A. R. Crofts. 1990b. Analysis of the pigment     content of an antenna pigment/protein complex from three strains of     Rhodopseudomonas sphaeroides. Biochim. Biophys. Acta. 502:409-416. -   Cogdell, R. J., A. M. Hawthomthwaite, M. B. Evans, L. A.     Ferguson, C. Kerfeld, J. P. Thornber, F. van Mourik, and R. van     Grondelle. 1990a. Isolation and characterisation of an unusual     antenna complex from the marine purple sulphur photosynthetic     bacterium Chromatium purpuraturn BW5500. Biochim. Biophys. Acta.     1019:239-244. -   Cogdell, R. J., and J. P. Thurber. 1979. The preparation and     characterization of different types of light-harvesting     pigment-protein complexes from some purple bacteria. In The CIBA     Foundation Symposium 61 (new series) on Chlorophyll Organisation and     Energy Transfer in Photosynthesis. G. Wolstenholme and D.     Fitzsimons, editors. Elsevier, Amsterdam. 61-79. -   Evans, M. B., A. M. Hawthomthwaite, and R. J. Cogdell. 1990.     Isolation and characterization of the different B800-850 light     harvesting complexes from low- and high-light grown cells of     Rhodopseudomonas cryptolactis. Biochim. Biophys. Acta. 1016:71-76. -   Yokthongwattana, K., et al. Isolation and characterization of a     xanthophyll-rich fraction from the thylakoid membrane of Dunaliella     salina (green algae), Photochem. Photobiol. Sci., 4:10288-1034,     2005. -   Mrquardt, J., et. al., Isolation and Characterization of Biliprotein     Aggregates of Acaryochlris marina, a Prochloron-like prokaryote     containing mainly chlorophyll d, FEBS Letters, 410:428-432, 1997. -   Adam G. Koziol, Tudor Borza, Ken-Ichiro Ishida, Patrick Keeling,     Robert W. Lee, and Dion G. Durnford, Tracing the Evolution of the     Light-Harvesting Antennae in Chlorophyll a/b-Containing Organisms,     Plant Physiology, April 2007, Vol. 143, pp. 1802-1816,     www.plantphysiol.org, 2007 American Society of Plant Biologists -   X. Hu, et al., Predicting the structure of the light-harvesting     complex II of Rhodospirillum molischianum, Protein Sci, 1995 4:     1670-1682. -   N Nagata, R Tanaka, S Satoh, J Minagawa, A Tanaka, Isolation and     characterization of a gene for chlorophyllide a oxygenase from     Prochlorothrix hollandica, Endocytobiosis Cell Res., 15: 321-327,     2004. -   Andreucii, F., et al., Isolation of phosphorylated and     dephosphorylated forms of the CP43 internal antenna of photosystem     II in Hordeum vulgare L. Journal of Experimental Botany 2005     56(414): 1239-1244. -   Andrew N. Webber, Rapid Isolation and Purification of Photosystem I     Chlorophyll-Binding Protein From Chlamydomonas reinhardtii,     Photosynthesis Research Protocols, Methods in Molecular Biology,     Volume: 274: 19-28, 2004. -   B R Green and D G Durnford, The Chlorophyll-Carotenoid Proteins of     Oxygenic Photosynthesis, Annual Review of Plant Physiology and Plant     Molecular Biology, Vol. 47: 685-714, 1996. Observation of the     Energy-Level Structure of the Low-Light Adapted B800 LH4 Complex by     Single-Molecule Spectroscopy, Biophysical Journal, 87: 3413-3420,     2004 -   Zhang, S-J, et al., Energy Transfer among Chlorophylls in Trimeric     Light-harvesting Complex II of Bryopsis corticulans, Acta Biochimica     et Biophysica Sinica 38(5): 310-317, 2006. -   Planck, Tracy, et al., Subunit Interactions and Protein Stability in     the Cyanobacterial Light-Harvesting Proteins, Journal of     Bacteriology, 177(12): 6798-6803, 1995. -   Bibby, T. S., et al., Low-light adapted Prochlorococcus species     possess specific antennae for each photosystem, Nature,     424:1051-1054, 2003

Reports:

-   Structure, Function and Reconstitution of Chlorosome Antennas from     Green Photosynthetic Bacteria, Robert E. Blankenship, Final Report     DE-FG03-01ER15214, DOE, September 2001-August 2004

Theses:

-   Hu, D. (2001) Investigation of the Fenna-Matthews-Olson protein from     photosynthetic green sulfur bacteria. Ph.D. Dissertation, Arizona     State University, Tempe, Ariz. -   LaBelle, J T, Design Feasibility of a Nanoscale Biophotonic Hybrid     Device, PhD Dissertation, Arizona State University, 2001. 

1. A hybrid photoactive device comprising: (a) a photoactive semiconductor; and (b) a plurality of chlorosomes supported in light communicating relation to a surface of the photoactive semiconductor.
 2. The hybrid photoactive device according to claim 1, wherein the chlorosomes comprise a cytoplasmic membrane sack of photosensitive molecular structures and a baseplate, and the plurality of cholorosomes comprises chlorosomes oriented with their baseplates facing the photoactive semiconductor and their cytoplasmic sack facing away from the photoactive semiconductor.
 3. The hybrid photoactive device according to claim 1, wherein the photoactive semiconductor has a light response that is diminished at a first range of light wavelengths, and the chlorosomes have a light response that is enhanced at a second range of light wavelengths that coincides, at least in part, with the first range of light wavelengths and an emission in the direction of the photoactive semiconductor of light outside the first range of light wavelengths.
 4. The hybrid photoactive device according to claim 3, wherein the photoactive semiconductor is a silicon photovoltaic cell.
 5. The hybrid photoactive device according to claim 1, wherein at least a majority of the chlorosomes are oriented in a light emitting direction toward the photoactive semiconductor.
 6. The hybrid photoactive device according to either claim 1 or 2, wherein the chlorosomes are RC chlorosomes of Chloroflexus aurantiacus (C. aurantiacus).
 7. The hybrid photoactive device according to claim 2, wherein the chlorosome baseplates are contiguous to a light receiving surface of the photoactive semiconductor.
 8. The hybrid photoactive device according to claim 2, wherein the chlorosome baseplates are spaced from the photoactive semiconductor.
 9. The hybrid photoactive device according to either claim 1 or 2, wherein the chlorosomes are adherent to a transparent plate overlying a light receiving surface of the photoactive semiconductor.
 10. The hybrid photoactive device according to claim 9, wherein the chlorosomes are adherent to a hydrophobic surface of the transparent plate.
 11. The hybrid photoactive device according to claim 10, wherein the plate is a borosilicate plate.
 12. The hybrid photoactive device according to claim 3, wherein the photoactive semiconductor diminished response is in a blue region of the visible spectrum and the chlorosomes respond to light thereon in the blue region of visible spectrum by emitting light outside the blue region.
 13. The hybrid photoactive device according to claim 12, wherein the light emitted by the chlorosomes is light in the 800 nm near infrared region of the visible spectrum.
 14. A method of making a hybrid photoactive device including: (a) providing photosynthetic chlorosome-containing bacteria, (b) extracting the chlorosomes from the bacteria, (c) providing a photoactive semiconductor, and (d) locating the chlorosomes proximate a light receiving surface of the photoactive semiconductor.
 15. The method according to claim 14, wherein step (d) comprises orienting at least the majority of chlorosomes in a light emitting direction toward the photoactive semiconductor.
 16. The method according to claim 14, wherein the chlorosomes comprise a cytoplasmic membrane sack of photo-sensitive molecular structures and a baseplate, and step (d) comprises orienting chlorosomes with their baseplates facing the photoactive semiconductor and their cytoplasmic sack facing away from the photoactive semiconductor.
 17. The method according to claim 14, wherein step (a) comprises providing Chloroflexus aurantiacus (C. aurantiacus).
 18. The method according to claim 17, wherein step (b) comprises extracting the RC⁻ chlorosome from the C. aurantiacus.
 19. The method according to claim 15, wherein orienting at least the majority of chlorosomes comprises locating the chlorosomes contiguous to a light receiving surface of the photoactive semiconductor.
 20. The method according to claim 16, wherein orienting chlorosomes comprises locating the chlorosomes with their baseplates contiguous to a light receiving surface of the photoactive semiconductor.
 21. The method according to claim 16, wherein step (d) comprises locating the chlorosomes spaced from and proximate the light receiving surface of the photoactive semiconductor.
 22. The method according to either claim 14 or 15, wherein step (d) comprises providing a transparent plate, securing the chlorosomes to the transparent plate and positioning the transparent plate overlying a light receiving surface of the photoactive semiconductor.
 23. The method according to claim 22, wherein providing the transparent plate further comprises providing a transparent plate having a hydrophobic surface and securing the chlorosomes further includes securing the chlorosomes to the hydrophobic surface.
 24. The method according to claim 23, wherein providing the transparent plate comprises providing a borosilicate plate.
 25. The method according to claim 14, wherein step (c) comprises providing a photoactive semiconductor having a light response that is diminished at a first range of light wavelengths, and step (a) comprises choosing a chlorosome having light response that is enhanced at a second range of light wavelengths that coincides, at least in part, with the first range of light wavelengths and light emission outside the first range of light wavelengths.
 26. A hybrid photoactive device made by the method of one of claims 14-25.
 27. A hybrid photoactive device comprising: (a) a photoactive semiconductor; (b) photoactive biological units extracted from photosensitive organisms; (c) the photoactive biological units having an enhanced response to light in a first range of light wavelengths to emit light prominent in light wavelengths in a second range of light wavelengths; (d) the photoactive semiconductor having enhanced photosensitivity to light in the second range of light wavelengths; and (e) the photoactive biological units supported and positioned to emit light towards the photosensitive semiconductor when illuminated by light in the first range of light wavelengths.
 28. A method of making a hybrid photoactive device including: (a) providing photosensitive organisms; (b) extracting from the photosensitive organisms photoactive biological units responsive to illumination by light in a first spectral region to emit light in a second spectral region; (c) providing a photoactive semiconductor responsive to illumination by light in the second spectral region; and (d) locating the photoactive biological units so as to illuminate the photoactive semiconductor by light emitted in the second spectral region when illuminated by light in the first spectral region. 